冯中红，王玉琴，杨成德，薛莉，陈秀蓉

(甘肃农业大学草业学院,草业生态系统教育部重点实验室,甘肃省草业工程实验室,

中-美草地畜牧业可持续发展研究中心,甘肃 兰州730070)

番茄细菌性叶斑病菌的拮抗菌筛选、鉴定及其拮抗性能评价

冯中红，王玉琴，杨成德*，薛莉，陈秀蓉

(甘肃农业大学草业学院,草业生态系统教育部重点实验室,甘肃省草业工程实验室,

中-美草地畜牧业可持续发展研究中心,甘肃 兰州730070)

摘要：采用含菌平板抑菌圈测定法，从120株高寒草地牧草内生细菌中分离筛选到一株对番茄细菌性叶斑病拮抗能力较强的菌株264ZY7，其抑菌圈直径为1.22 cm。264ZY7菌体短杆状，大小为1.534 μm×0.571 μm~3.210 μm×0.781 μm，革兰氏阳性菌，根据形态特征，并结合16S rDNA和gyrB基因序列相似性分析，将菌株264ZY7鉴定为解淀粉芽孢杆菌Bacillus amyloliquefaciens。通过室内盆栽防效试验，结果表明该菌株对番茄细菌性叶斑病的防效达69%，此外，该菌株对8种病原真菌具有抑菌能力，说明抑菌谱较宽，且抑菌率均大于30%，对番瓜根腐病菌(Fusarium sp.)效果最明显，抑菌率为52.84%。该研究结果为高寒草地紫花针茅内生细菌264ZY47的进一步开发利用提供了依据。

关键词：番茄叶斑病菌；拮抗内生细菌；筛选；鉴定；防效

DOI：10.11686/cyxb2014450http://cyxb.lzu.edu.cn

冯中红， 王玉琴， 杨成德， 薛莉， 陈秀蓉. 番茄细菌性叶斑病菌的拮抗菌筛选、鉴定及其拮抗性能评价. 草业学报, 2015, 24(8): 166-173.

Feng Z H, Wang Y Q, Yang C D, Xue L, Chen X R. Screening， identification and assessment of endophytic bacteria antagonistic totomato bacterial spot. Acta Prataculturae Sinica, 2015, 24(8): 166-173.

收稿日期：2014-11-03；改回日期：2014-12-10

基金项目：甘肃省蔬菜攻关项目(No.GJ201307) 资助。

作者简介：冯中红(1991-),女,甘肃临夏人,在读硕士。E-mail:1142595813@qq.com

通讯作者*Corresponding author. E-mail:yangcd@gsau.edu.cn

Screening， identification and assessment of endophytic bacteria antagonistic totomato bacterial spot

FENG Zhong-Hong, WANG Yu-Qin, YANG Cheng-De*, XUE Li, CHEN Xiu-Rong

CollegeofGrassland,GansuAgriculturalUniversity,KeyLaboratoryofGrasslandEcosystemGansuAgriculturalUniversity,MinistryofEducation,PrataculturalEngineeringLaboratoryofGansuProvince,Sino-U.S.CenterforGrazingLandEcosystemSustainability,Lanzhou730070,China

Abstract:After screening by assessing inhibition zones on nutrient media an antagonistic endophytic bacteria, strain 264ZY7, was identified as having strong antagonistic ability against Pseudomonas syringe pv. tomato which causes bacterial spot on tomatoes. This strain was selected from 120 endophytic bacteria strains isolated from alpine meadow pasture. Strain 264ZY7 produced a 1.22 cm diameter inhibition zone. The bacteria is rod-shaped with a spore size of 1.534 μm×0.571 μm-3.210 μm×0.781 μm, gram-positive and was identified as Bacillus amyloliquefaciens based on morphology and homology analysis of the 16S rDNA and gyrB gene sequence. The control efficacy of strain 264ZY7 on bacterial spot on tomatoes grown in pots ranged up to 69%. It was also able to control fungal pathogens, in particular Fusarium sp. (52.84% efficacy). These results suggest that further development and utilization of this endophytic bacteria strain (264ZY7) should be undertaken.

Key words:Pseudomonas syringe; antagonistic endophytic bacteria; screening; identification; control effect

番茄细菌性叶斑病(番茄细菌性斑点病)(Pseudomonassyringapv.tomato)主要……