静态顶空-气相色谱法用于柳叶蜡梅挥发油指纹图谱研究

梁现蕊，肖钦钦

(浙江工业大学 药学院，浙江 杭州 310014)

摘要：建立柳叶蜡梅挥发油的气相色谱指纹图谱分析方法,为全面控制柳叶蜡梅质量提供参考.采用HP-INNOWAX毛细管柱(30 m × 0.25 mm × 0.50 μm)，顶空平衡温度80 ℃，顶空平衡时间50 min，进样口温度200 ℃，检测器温度250 ℃，程序升温静态顶空-气相色谱(HS-GC)分析条件，对12批柳叶蜡梅样品进行指纹图谱分析，标定了25个共有峰,结合相似度软件、聚类分析和主成分分析对12批样品进行系统的比较与归类,将不同批次的柳叶蜡梅样品分为两类.该方法精密度好，简单快速，为柳叶蜡梅品种的鉴定与质量评估提供了可靠的依据.

关键词：柳叶蜡梅；静态顶空-气相色谱(HS-GC)；指纹图谱；挥发油；鉴别

收稿日期：2015-03-26

基金项目：国家自然科学基金资助项目(21206148)

作者简介：梁现蕊(1975—)，女，山东临沂人，副教授，博士，主要从事药物结构分析研究，E-mail: liangxrvicky@zjut.edu.cn.

中图分类号：R284.1

文献标志码：A

文章编号：1006-4303(2015)05-0567-06

Abstract:This study aimed to establish a fingerprints analysis method of volatile oil in Chimonanthus salicifolius for quality control by static headspace-gas chromatography (HS-GC). HS-GC analysis was performed on the HP-INNOWAX capillary column (30 m × 0.25 mm × 0.50 μm) with the inlet temperature at 200 ℃ and the FID detector temperature at 250 ℃. The HS oven temperature was 80 ℃, and the vial equilibration time was 50 min. The optimized method was applied to the fingerprints analysis of 12 samples and 25 characteristic peaks were picked out. The data was analyzed by chemometrics methods such as similarity analysis, hierarchical cluster analysis and principal component analysis. 12 samples were divided into 2 groups. A new, rapid and simple HS-GC fingerprint method was developed. It provides a novel strategy for the origin distinction and quality evaluation of Chimonanthus salicifolius.

Keywords:Chimonanthus salicifolius; static headspace-gas chromatography (HS-GC); fingerprints; volatile compounds; authentication

Research on the fingerprints of volatile oil inChimonanthussalicifolius

by static headspace-gas chromatography

LIANG Xianrui, XIAO Qinqin

(College of Pharmaceutical Science, Zhejiang University of Technology, Hangzhou 310014, China)

柳叶蜡梅(Chimonanthussalicifolius)系蜡梅科蜡梅属半常绿灌木川，在我国具有悠久的栽培历史和药用价值，其产地主要分布于我国江西和浙江等省[1].用柳叶蜡梅的叶片和嫩芽为原料制成的绿茶，在浙江丽水和江西婺源一带被称作“食凉茶”或“黄金茶”，具有抗菌，消炎等作用，同时对防治感冒、喉咙肿痛、慢性气管炎等也有较好的疗效[2-4].此外，柳叶蜡梅中含有丰富的挥发油成分，因此它将是精油生产的一个重要来源[4-5].然而，对于蜡梅属品种的分类研究，一直以来都存在不同的……