BAPTA-AM脂质体抗大鼠重症急性胰腺炎的作用

宋必卫，郑彩云，俞巧丽

(浙江工业大学 药学院，浙江 杭州 310014)

摘要：为观察BAPTA-AM脂质体(BA-L)抗重症急性胰腺炎(SAP)的作用，采用胰胆管逆行注射4%牛黄胆酸钠(TC)制作大鼠SAP模型.BA-L治疗组于造模后立即尾静脉注射相应剂量BA-L，给药24 h后，观察各组大鼠的死亡情况，检测各组大鼠血清AMS，LDH，TNF-α水平以及胰腺组织匀浆MDA水平及SOD活性，HE染色观察胰腺组织病理学改变.结果表明：给予BA-L治疗后SAP大鼠死亡率明显降低，血清AMS，LDH，TNF-α水平和胰腺组织匀浆MDA水平显著降低，SOD活性增高，HE染色组织切片显示BA-L明显减轻胰腺组织损伤.表明BA-L能显著减轻大鼠SAP，为开发BA-L成临床SAP治疗新药物提供必要的药效学依据.

关键词：BAPTA-AM；急性胰腺炎；Ca`(2+)；TNF-α

收稿日期：2015-04-17

作者简介：宋必卫(1956—)，男，安徽岳西人，教授，研究方向为神经分子药理学，E-mail:bwsong@zjut.edu.cn.

中图分类号：R965

文献标志码：A

文章编号：1006-4303(2015)05-0562-05

Abstract:To explore the protective effect of BAPTA-AM liposome (BA-L) on severe acute pancreatitis (SAP), a rat SAP model was established by retrograde injection of 4% sodium taurocholate (TC) into pancreatic duct. After modeling, BA-L groups were immediately administered BA-L in different doses. After 24 h of BA-L administration, the mortality of rats, serum AMS, LDH and TNF-α levels, tissue MDA and SOD levels were detected. Pathological changes of pancreatic tissue were examined. The results show that BA-L could significantly reduce the mortality compared to the modeling. A comparison of the modeling groups shows that the AMS, LDH, TNF-α and MDA levels of the BA-L group were reduced remarkably, however, the SOD activity was increased significantly. These changes were also associated with parallel changes in histopathological appearance. In conclusion: The results above demonstrated that BA-L can exert an admirable protection against TC induced SAP in rats. The study also provides the essential therapeutic basis for BA-L to be developed as an anti-SAP drug.

Keywords:BAPTA-AM; acute pancreatitis; Ca`(2+); TNF-α

Protective effect of BAPTA-AM liposome on severe acute pancreatitis in rats

SONG Biwei, ZHENG Caiyun, YU Qiaoli

(College of Pharmaceutical Science, Zhejiang University of Technology, Hangzhou 310014, China)

急性胰腺炎是临床较为多见的急腹症之一，是由多种病因引发的胰酶在胰腺内部被异常激活后，继发胰腺组织自身消化、水肿、出血甚至坏死的一系列炎症反应.其发病率呈逐年上升趋势，大部分急性胰腺炎患者病情具有自限性，预后良好；但有20%～30%的患者可进展为重症急性胰腺炎(Severe acute pancreatitis，SAP)，SAP可引发全身炎症反应综合征(Systemic inflammatory response syndrome，SIRS)，以致多器官功能障碍综合征(Multiple organ dysfunction syndrome，MODS)，甚至是多器官功能衰竭(Multiple system organ failure，MSOF)，从而导致死亡[1-2].目前，临床SAP治疗效果差，仍缺乏针对发病因素、能很好保护胰腺细胞特效治疗药物，SAP病死率仍然高达10%～30%[3].SAP的发病机制极其复杂，诱因诸多，如胆道疾病、内分泌……