尼古丁降解菌株HZN7的最佳生长条件及土壤中降解特性

马云，洪骏

(浙江工业大学 生物与环境工程学院，浙江 杭州 310014)

摘要：利用从杭州农药厂活性污泥中筛选到的一株新型高效降解尼古丁的菌株Shinella sp.HZN7为对象，通过正交试验对影响菌株生长的pH值、温度、氯化钠、葡萄糖、转速等条件参数进行了优化，得出了其在实验室条件下的最佳生长条件.通过在土壤中添加烟草废弃物，利用该高效尼古丁降解菌株Shinella sp.HZN7对模拟烟草废弃物污染土壤进行生物修复的土壤实验，实验表明：添加菌株的处理组与不加菌株的对照组相比，在尼古丁的去除能力、pH值稳定性、含水率保持等方面均有较大改善，以及对土壤有机质量的变化产生一定的影响，且在一定范围内，随着烟草废弃物添加量的增大，生物修复的效果越明显.

关键词：尼古丁；正交实验；降解；生物修复

收稿日期：2015-03-27

基金项目：国家自然科学基金资助项目(21007058)；浙江省自然科学基金资助项目(GB13031050069)

作者简介：马云(1977—)，女，浙江杭州人，副教授，博士，研究方向为环境微生物，E-mail:mayun@zjut.edu.cn.

中图分类号：X172

文献标志码：A

文章编号：1006-4303(2015)05-0537-06

Abstract:Taking a strain of bacterium Shinella sp. HZN7 degrading nicotine isolated from the sludge in insecticide factory of Hangzhou as an object, we determined its optimal growth conditions by orthogonal experimental design. The factors are including pH value, temperature, sodium chloride, glucose and the speed of shaking tables. By adding tobacco waste into soil, the soil experiment includes that the bioremediation of the soil contaminating tobacco waste by using the degrading bacterium Shinella sp. HZN7, it indicates that the nicotine removal efficiency was better than the case in which the bacterium Shinella sp. HZN7 was not added. In addition, pH stability with time and the moisture content was improved, the total organic carbon was influenced. The effect of bioremediation was more remarkable with adding more tobacco waste within its limits.

Keywords:nicotine; orthogonal experiment; degradation; bioremediation

Optimal growth conditions and degrading nicotine characteristic

in soil of strain HZN7

MA Yun, HONG Jun

(College of Biological and Environmental Engineering, Zhejiang University of Technology, Hangzhou 310014, China)

尼古丁，又名烟碱，是一种存在于茄科植物中的生物碱，也是烟草的重要成分[1].由于尼古丁水溶性很大，能通过口、鼻、支气管黏膜被人体迅速吸收，进入人体后能使人上瘾或产生依赖性.重复使用会对心、脑、肺和血管等造成损伤，严重的还会引发肺癌等在内的多种疾病[2].对比于传统的物理、化学处理法，利用微生物降解菌……