缩泉胶囊对自然衰老大鼠尿量及尿中离子浓度的影响

吴君1，韩芸2， 王志江1，黄萍3*

(1.山东中医药高等专科学校， 烟台264199；2.滨州医学院， 烟台264003；3广州中医药大学， 广州 510006)

摘要：目的观察缩泉胶囊对自然衰老大鼠24 h尿量、水负荷后5 h尿量及尿中Na`+、K`+、Cl`-浓度的影响。方法取老年大鼠(20月龄)50只，青年大鼠(3月龄)10只。设置组别为：空白对照组、模型对照组、金匮肾气丸组、缩泉胶囊高、中、低剂量组。用代谢笼法测量大鼠24 h尿量及水负荷5 h尿量；用生化分析仪测定尿液中Na`+、K`+、Cl`-浓度。结果缩泉胶囊中、高剂量组24 h尿量明显减少；缩泉胶囊低、中、高剂量组水负荷后5 h内尿量明显减少；缩泉胶囊低、中、高剂量组尿液中Na`+、Cl`-排出量明显减少(P<0.05～0.01)；缩泉胶囊中、高剂量可以显著增加自然衰老大鼠尿液中K`+的排出量(P<0.05)。结论自然衰老大鼠多尿的发生可能与Na`+、K`+及Cl`-排泄异常有关，而缩泉胶囊可使“肾虚多尿”大鼠尿Na`+、Cl`-排出减少，尿K`+排出增加，同时尿量明显减少，表明其抗利尿作用可能是通过保钠排钾的作用实现的。

关键词:缩泉胶囊；尿量；离子浓度

doi:10.3969/j.issn.1004-2407.2015.03.014

中图分类号：R965

文献标志码：A

文章编号：1004-2407(2015)03-0264-03

Abstract:ObjectiveTo study the effects of Suoquan Capsules(SQC) on the 5 h urine volume and Na`+， K`+， Cl`- concentration in natural aging rats. Method10 young SD rats were chosen as a control and 50 aging SD rats (20 months) were randomly divided into five groups: model， Jinkuishenqi Pills group， SQC with high, middle and low dose groups.The urine volume at 5 h after the water loaded in rats and at 24 h urine were measured in metabolic cages. The Na`+， K`+， Cl`- concentration in rat urine were measured by a biochemistry analyzer. Results Compared with the control group, the 24 h urine volume of SQC with middle and high dose significantly decreased，while the 5 h urine after the water loaded amount of SQC with low，middle and high dose group also significantly decreased. The discharged of Na`+,Cl`- significantly decreased in rats with high，middle and low dose of SQC after intragastric administration(P<0.05-0.01). SQC with high and middle dose significantly increased the discharged amounts of K`+ in urine of natural aging rats(P<0.05). Conclusion Polyuria in the natural aging rats was possibly related to the excretion of abnormal Na`+， K`+ and Cl`-. SQC can reduce Na`+， Cl`- emission and increase K`+ excretion in kidney deficiency rats with polyuria and has antidiuretic effect by storing Na`+ and excreting K`+.

作者简介：吴君，男，讲师，博士

收稿日期：(2014-09-09)

Effects of Suoquan Capsules on the urine volume and iron concentration in natural aging rats

WU Jun1， HAN Yun2，WANG Zhijiang1，HUANG Ping3*(1.Shandong College of Traditional Chinese Medicine，Yantai 264199，China；2.Binzhou Medical University, Yantai 264003，China； 3.Guangzhou University of Chinese Medicine，Guangzhou 510006，China)

*通信作者：黄萍，女，教授，博士生导师

缩泉丸出自《妇人良方》，由乌药、益智仁、山药组成，具有温肾祛寒、缩尿止遗的功效，主治膀胱虚寒证，其组方科学[1]。现代临床常加减运用或者联合其他药物使用，用于治疗小儿遗尿、小儿夜尿、神经性尿频、尿失禁、尿道综合征、尿崩症及多尿等疾病[2-6]。缩泉胶囊是由缩泉丸改剂型的制剂。本课题组从缩泉胶囊对肾虚多尿模型的尿量、尿中离子浓度的影响进行研究，初步探讨其作用机制，为后续的研究奠定药效学基础。

1仪器与材料

1.1仪器日立7180型生化分析仪(日本日立公司产品)；JA1203N型电子天平(上海精密仪器有限公司产品)。

1.2药物缩泉胶囊(湖南汉森制药有限公司，批号100302)；……