低氧下大鼠AQP5基因差异性表达※

2016-01-19冯喜英安文静刘洪千

中国高原医学与生物学杂志 2015年2期

关键词：肺水肿

久　太，冯喜英，安文静，刘洪千

(青海大学附属医院呼吸内科，西宁 810001)

久太(1971～)，男，藏族，青海籍，副主任医师

低氧下大鼠AQP5基因差异性表达※

久太，冯喜英，安文静，刘洪千

(青海大学附属医院呼吸内科，西宁 810001)

摘要目的检测低氧下大鼠肺组织AQP5 表达水平的改变，探讨AQP5表达量变化与低氧下肺水肿发病的分子机制。方法构建低氧大鼠模型；采用荧光定量PCR技术检测低氧下大鼠肺组织中AQP5 mRNA表达水平；采用Western-Blot技术检测低氧下大鼠肺组织中AQP5 蛋白表达水平；并与平原对照组进行表达差异性比较。结果低氧组大鼠肺组织中AQP5 mRNA表达水平随着时间的延长升高，其中72 小时组 AQP5 mRNA表达水平达到最高，且差异极为显著(P<0.01)；并且低氧组大鼠肺组织中 AQP5 蛋白表达水平变化与其 mRNA 表达水平变化基本一致。结论AQP5 在机体高原习服过程中起到了重要的调节过程；AQP5 对肺泡性肺水肿发生起代偿作用；机体进入高原环境，肺组织中 AQP5 表达量最高值可作为机体发生高原肺水肿的诊断标准。

*：※：青海大学附属医院青年基金项目(ASRF-2012-08)

关键词低氧大鼠AQP5差异

中图分类号R555

文献标识码识码A

DOI：10.13452/j.cnki.jqmc.2015.02.008

AbstractObjectiveTo study the mRNA expression levels of AQP5 in lung tissue of rats with acute，chronic exposed hypoxia in order to clarify the relationship between the expression changes of AQP5 and pulmonary edema，to provide the theoretical basis for the study of high altitude adaptation mechanisms.Methods The acute，chronic exposed hypoxic rat model were set up，at the same time，we observed the change of the expression level of AQP5 mRNA in lung tissue by quantitative PCR in the acute and chronic hypoxia；then the AQP5 protein expression levels were detected by Western-Blot.Results the mRNA expression levels of AQP5 genes were increased significantly both in the acute and chronic hypoxia group(P<0.01).In addition，after 72 hour AQP5 mRNA expression level has reached the significant highest levels(P<0.001)；AQP5 protein expression level was consistent with the mRNA levels.Conclusion AQP5 plays important role in adaptation mechanisms in high altitude；AQP5 also play a compensatory role in the pathogenesis of the pulmonary edema when exposed to acute and chronic hypoxia，it might also be a potential diagnosis values for the severity of the high altitude pulmonary edema.

KeywordsHypoxiaRatAquaporin 5Difference

收稿日期2014-08-09

HYPOXIA EXPOSURE CAUSED DIFFERENT

AQP5 GENE EXPRESSIONS IN RATS

Jiu Tai，Feng Xiying，An Wenjing，Liu Hongqian

(Respiratory Department of Internal Medicine，Affiliated Hospital of Qinghai University，Xining，Qinghai 810001)

当人体进入海拔3000 m 以上后，个别急性高原反应者将发生高原肺水肿 (HAPE)[1]。水通道蛋白5(AQP5)主要作用是参与水的转运，维持细胞内外水平衡，并参与调节肺脏粘液、唾液分泌[2]。AQP5的调节方式分为长时调节和短时调节：长时调节是指在基因转录时相AQP生成增多，引起mRNA和蛋白质高表达[3]。短时调节是指AQP 在所处机体内环境(如PH值，渗透压等)发生改变时导致AQP活性或功能性水通道的数目发生变化[4]。目前已知肺部感染、慢性阻塞性肺疾病(Chronic obstructive pulmonary disease，COPD)、肺癌、哮喘、肺水肿等多种疾病的发病均与AQP5 的表达改变及功能异常有关[4]。因此，AQP5 可能与肺部疾病存在着某种关联。

1材料与方法