大鼠肝脏半乳糖凝聚素-3 cDNA分子多样性分析

方恩浩1，2，张珊珊3，刘倩芸3，周建钟4，杨仙玉1*

(1.浙江农林大学动物科技学院,浙江 临安 311300;

2. 浙江农林大学集贤学院,浙江 临安 311300;

3. 浙江农林大学林业与生物技术学院,浙江 临安 311300;

4. 浙江省林业生物质化学利用重点实验室，浙江 临安 311300)

摘要：半乳糖凝聚素-3(Galectin-3，Gal-3)是一种多功能蛋白，参与机体内的多种生命活动过程。为分析大鼠Rattus norvegicus gal-3 cDNA分子多样性，在分析GenBank注册的大鼠gal-3 cDNA 序列的同时，从三只不同大鼠个体的肝脏第一链cDNA，共计9个独立实验中克隆gal-3 开放阅读框OFR (Open reading frame)。分析结果表明，在已注册的序列中存在1个单核苷酸多态性 (Single nucleotide polymorphism，SNP)。本实验从1-3号大鼠肝脏分别获得118、95和120个克隆，新发现1处缺失和5处SNP，共克隆17个不同的 gal-3 ORF，其中仅1个克隆与已报告大鼠gal-3 cDNA 的ORF完全相同。从1号和3号个体肝脏中分别克隆11和14个不同的gal-3 ORF，揭示大鼠gal-3 cDNA存在个体水平的分子多样性。

关键词：大鼠；半乳糖凝聚素-3；cDNA多样性；单核苷酸多态性

中图分类号：S443.5;Q533+.3文献标志码：A

收稿日期：2015-01-22;修回日期：2015-04-15.

作者简介：宋东光，男，副教授，研究方向:生物信息学与植物分子育种;E-mail:3dsong@163.com.

doi:10.3969/j.issn.1672-5565.2015.02.04

Livergalectin-3 cDNA diversity inRattusnorvegicus

FANG Enhao1，2, ZHANG Shanshan3，LIU Qianyun3，ZHOU Jianzhong4，YANG Xianyu1*

(1.CollegeofAnimalScienceandTechnology,ZhejiangAgriculturalandForestryUniversity,Lin′an311300,China;

2.JixianHonorsCollege,ZhejiangAgriculturalandForestryUniversity,Lin′an311300,China;

3.SchoolofForestryandBiotechnology,ZhejiangAgriculturalandForestryUniversity,Lin′an311300,China;

4.ZhejiangProvincialKeyLaboratoryofChemicalUtilizationofForestryBiomass, 88HuanchengBeilu,Lin′an311300,China)

Abstract：Galectin-3 (Gal-3) is a multi-functional protein involved in many biological processes. To clarify the gal-3 cDNA diversity of Rattus norvegicus, cloning of gal-3 open reading frame (ORF) from liver first strand cDNA of three individuals was performed totally from 9 independent experiments. Consequently, 118, 95 and 120 clones were obtained from three individuals, respectively. Alignment of all these sequences indicated 1 deletion and 5 single nucleotide polymorphism (SNP) sites, and 17 different clones were obtained. Nine out of the 17 clones appeared in two or more independent experiments, and 11 and 14 different clones were obtained from the first and the third individuals, respectively. These results indicated the objectivity of liver gal-3 cDNA diversity at individual level in R. norvegicus.

Keywords：Rattus norvegicus; Galectin-3; cDNA diversity; SNP

近年来，高通量DNA测序技术的发展，基因组和转录组的数据在不断的积累，学者们逐渐发现转录子的多样性以及转录产物 (RNA)与基因组DNA不相匹配的现象，而且这种多样性出现于个体水平[1-2]。半乳糖凝集素(Galectins, Gal)作为动物类凝聚素，通过其高度保守的糖识别结构域 (Carbohydrate recognition domain, CRD)特异性识别并结合β-半乳糖苷，从而发挥其生物学功能[3]。在哺乳类，已发现15个家族成员，分为原型、串联重复型和嵌合型三类[3]。……