斑马鱼CYP11a1基因在不同性腺发育时期的表达

陈孝红，仇雪梅，郝薇薇，王秀利

(大连海洋大学 农业部北方海水增养殖重点实验室，辽宁 大连 116023)

摘要:采用实时荧光定量PCR技术，分析了斑马鱼Danio rerio的CYP11a1基因在成鱼4个组织和性腺发育3个时期的表达情况。结果表明：CYP11a1基因在卵巢、精巢、肌肉和脑组织中均有表达且存在差异，在脑中表达量最低(P<0.05)，在卵巢、精巢、肌肉中的表达量分别为脑中表达量的204.7倍、38.9倍、17.0倍;CYP11a1基因在3个卵巢发育时期的表达量表现为先降低后升高的趋势，其在卵巢成熟期的表达量最低(P>0.05)，在卵巢成熟前期、卵巢成熟后期的表达量分别为卵巢成熟期表达量的1.7倍、1.5倍；CYP11a1基因在3个精巢发育时期的表达量表现为先升高后降低的趋势，在精巢成熟期表达量最高(P<0.05)，分别为精巢成熟前期、精巢成熟后期表达量的6.5倍、13.4倍。研究表明，尽管CYP11a1基因在卵巢和精巢发育过程中的表达规律不同，但CYP11a1基因在一定程度上与斑马鱼的性腺发育相关，其可能在促进斑马鱼精子成熟中发挥一定作用。

关键词:斑马鱼；CYP11a1基因；性腺发育

DOI：10.3969/J.ISSN.2095-1388.2015.01.003

文章编号：2095-1388(2015)01-0013-05

中图分类号：Q45

文献标志码：A

收稿日期：2014-04-29

基金项目：国家海洋公益性行业科研专项(201405003)

作者简介：陈孝红(1988—)， 女， 硕士研究生。E-mail:xh_chen2011@163.com

Abstract：The expression of CYP11a1 gene was studied and analyzed in four tissues and three development phases in gonad of zebrafish Danio rerio by quantitative Real-time PCR (qRT-PCR). It was found that the CYP11a1 gene was expressed in ovary, testis, muscle and brain, the minimum in brain(P<0.05)， 204.7 times higher in ovary, 38.9 times higher in testis, and 17.0 times higher in muscle compared with that in brain, indicating that the CYP11a1 gene showed a certain extent tissue difference in expression. The qRT-PCR analysis revealed that the expression of CYP11a1 gene was found to be decreased first in the early development phases of ovary and then enhanced in the later development phases of ovary, the minimum at maturity phase(P>0.05), 1.7 times higher before sexual maturity and 1.5 times higher after sexual maturity compared with that at maturity phase. In the testis, however, the expression of CYP11a1 gene was found to be enhanced first in the early development phases of testis and then decreased in the later development phases of testis, the maximum at the sexual maturity(P<0.05), 6.5 times higher before sexual maturity and 13.4 times higher than that after the sexual maturity. The findings indicated that the expression of CYP11a1 gene was involved in gonad development and spermatogenesis in zebrafish, though the expression pattern of CYP11a1 gene was different in the ovary and the testis.

斑马鱼Daniorerio隶属于鲤科、短担尼鱼属，是一种具有较高经济价值和观赏价值的热带观赏鱼。由于其具有果蝇、小鼠等模式生物不可比拟的优点，如繁殖能力强、体外受精、胚胎透明、发育快、性成熟周期短、可追踪整个胚胎发育过程等，因此，其特别适合作为研究生长与发育、性别决定、性别分化相关基因表达与功能的模式生物。目前，斑马鱼已被广泛应用于遗传学、生殖发育生物学、生态学等[1]研究中。

细胞色素P450(Cytochrome P450,CYP)基因家族是一类编码参与一些内源性物质(如激素、胆固醇)和外源性物质(如药物、环境化合物)代谢的酶系超基因家族[2]。由CYP基因编码的CYP450酶属于血红蛋白酶类，是一类以血红素为辅基的细胞色素蛋白酶超家族，广泛存在于生物体内。……