紫椴种皮透性及种子浸提物生物效应的研究

2016-01-12张洁,杨立学,王海南等

森林工程 2015年2期

紫椴种皮透性及种子浸提物生物效应的研究

张洁1，杨立学1*，王海南2，王会仁1

(1.东北林业大学林学院，哈尔滨 150040；2.中南大学宣传部，长沙 410000)

摘要：采用称重和生物测定的方法，对紫椴种皮透水性及种子各部分浸提物的生物效应研究，实验结果表明，紫椴种子种皮透水性较差，不易吸水。紫椴种子的胚乳、种皮、果皮中确实都含有萌发抑制类的物质，按照其抑制强度从大到小依次为种皮、果皮、胚乳(胚)，且该抑制物质浸提液浓度高则抑制性强，浸提液浓度低则抑制性弱。紫椴种子胚各有机相及水相对白菜种子发芽率的抑制活性从大到小依次为：乙酸乙酯相，石油醚相，乙醚相，水相，甲醇相；对白菜种子胚根长抑制活性从大到小依次为：石油醚相，水相，乙酸乙酯相，乙醚相，甲醇相。种皮的各有机相及水相对白菜种子发芽率和胚根长的抑制活性从大到小依次为：甲醇相，水相，乙醚相，乙酸乙酯相，石油醚相。果皮的各有机相及水相对白菜种子发芽率和胚根长的抑制活性从大到小依次为：甲醇相，乙酸乙酯相，石油醚相，乙醚相，水相。

关键词：紫椴；种皮透性；种子浸提物；生物测定

中图分类号：S 792.36

文献标识码：A

文章编号：1001-005X(2015)02-0001-04

Abstract：The testa permeability of Amur linden seed and biological effect of seed extract were studied using the weight and biological assay methods.Results showed that the testa had poor permeability for the waterproof compact layer.There was germination inhibitors in the pericarp，testa and endosperm(embryo)and the inhibitory activities was ordered as testa>pericarp>endosperm(embryo)，which had a positive correlation with the concentration.The inhibitory activities for the percentage germination of B.pekinensis seedlings of all organic phases and water phase in the embryo was ordered as follows：ethyl acetate phase，petroleum ether phase，ethylether phase，water phase，methanol phase；while the order for the root growth of B.pekinensis seedlings was petroleum ether phase，water phase，ethyl acetate phase，ethylether phase，and methanol phase.The inhibitory activities for the percentage germination of B.pekinensis seedlings and root growth of all organic phases and water phase in the testa orders were methanol phase，water phase，ethylether phase ethyl acetate phase，and petroleum ether phase.For the pericarp，the inhibitory activity orders were methanol phase，ethyl acetate phase，petroleum ether phase，ethylether phase，and water phase.

Keywords：tilia amurensis；testa permeability；seed extract；biological assay

收稿日期：2014-10-11

基金项目：黑龙江省林业科技项目( GA09B202-06)

作者简介：第一张洁，硕士研究生。研究方向：森林培育。

通讯作者：*杨立学，博士，教授。研究方向：林木间化感作用。E-mail：ylx_0813@163.com

Water Permeability of Seed Coat and Bio-effectof Seed Extraction Material ofTiliaamurensis

Zhang Jie1，Yang Lixue1*，Wang Hainan2，Wang Huiren1

(1.School of Forestry，Northeast Forestry University，Harbin 150040；

2.Department of Propaganda，Central South University，Changsha 410000)