李龙昱(1978～)，男，汉族，甘肃籍，主管药师

唐古特大黄多糖对RAW264.7巨噬细胞免疫调节作用的影响

李龙昱

(青海省人民医院药学部)

摘要目的研究唐古特大黄多糖对RAW264.7巨噬细胞免疫功能的调节作用。方法以小鼠腹腔巨噬细胞系RAW264.7为研究对象，分不同浓度的唐古特大黄多糖实验组、脂多糖阳性对照组、不加任何样品的空白对照组。通过MTT实验、吞噬中性红实验、一氧化氮释放量及细胞因子(IL-1β、IL-6、IFN-α、IFN-γ)的分泌水平，研究唐古特大黄多糖的免疫调节作用。结果实验表明唐古特大黄多糖在一定浓度范围可以促进吞噬细胞的增殖，并可增强其吞噬作用；唐古特大黄多糖可以有效提高巨噬细胞产生NO的量，且对巨噬细胞的细胞因子IL-1β、IL-6、IFN-α、IFN-γ的分泌量均有一定程度的提高。结论唐古特大黄多糖具有较好的提高巨噬细胞免疫功能的作用。

关键词唐古特大黄巨噬细胞免疫调节

中图分类号R967

文献标识码A

DOI：10.13452/j.cnki.jqmc.2015.03.010

AbstractObjectiveTo investigate the influence of rheum tanguticum polysaccharides on the immunomodulatory effectsofRAW264.7macrophage.Methods To take murineintraperitonealRAW264.7macrophage as the research objects and different concentrations of rheum tanguticum polysaccharides and LPS injected into mice as positive control，without any treating asthe blank control.The influence of rheum tanguticum polysaccharides on the immunomodulatory effectsofRAW264.7macrophage was detected by MTT，phagocytosis test，NO release and the secretion of cytokines such as IL-1β，IL-6，IFN-α and IFN-γ.Results Experiments showed that rheum tanguticum polysaccharides can accelerate the proliferation of phagocytic cells to have a good phagocytosis.Besides，it can improve the macrophage to generate NO，and had a good promotion in enhancing the secretion of cytokines such as IL-1β，IL-6，IFN-α and IFN-γ.Conclusion Rheum tanguticum polysaccharides can obviously enhance the function of macrophage.

KeywordsRheum tanguticumMacrophagesImmunomodulatory effect

收稿日期2015-04-12

THE INFLUENCE OF RHEUM TANGUTICUM

POLYSACCHARIDES ON THE IMMUNOMODULATORY

EFFECTS OF RAW264.7MACROPHAGE

Li Longyu

(The Pharmaceutical Department of the People's Hospital of Qinghai Province)

唐古特大黄(R.tanguticumMaxim.exBalf.)是大黄药材的重要基原，《中国药典》收录的3种大黄基原中以唐古特大黄质量最优[1]。唐古特大黄为青海省的道地药材，临床主要用于治疗肾衰竭、重症急性胰腺炎、萎缩性胃炎等[2]。中药多糖在免疫调节方面展示出较好的潜力，受到越来越多的关注[3，4]。有文献报道大黄多糖具有免疫调节的作用[5]。故本研究以小鼠腹腔巨噬细胞系RAW264.7为研究对象，检测唐古特大黄多糖对巨噬细胞的体外免疫调控作用，为唐古特大黄多糖的综合开发和利用提供一定的参考依据。……