※：青海省科技项目应用基础研究计划(2012-Z-727)

郜茜(1975~)，女，汉族，青海籍，副主任医师

Ack-1和CDC42在胃癌组织中的表达和意义

郜茜，卢永福，王昀，杨永成，蒋汉梅

(青海大学附属医院消化内科)

摘要目的探讨胃癌组织和癌旁组织中Ack-1、CDC42基因和蛋白的表达水平，了解两者在胃癌组织中的表达特点和相关性及对胃癌临床病理特点的影响。方法于胃癌病变处取2～4块黏膜活检，并在离癌旁大于5 cm处取正常组织1～2块。采用实时定量PCR(real-time PCR)和免疫印记(Western-blot)法检测胃癌组织和癌旁组织中Ack-1、CDC42基因和蛋白的表达量，并行相关分析。结果胃癌组织中Ack-1、CDC42 mRNA表达较癌旁组织明显增多，差异有统计学意义(P<0.01)，CDC42的表达与肿瘤浸润深度有关(P<0.01)；Ack-1、CDC42的表达呈明显正相关(r=0.481，P<0.01)。胃癌组织中Ack-1、CDC42蛋白量的表达也较癌旁组织明显升高，分别为(2.44±0.45)vs(1.34±0.24)(t=4.349，P<0.05)和(2.81±0.45)vs(1.37±0.31)(t=4.755，P<0.05)。结论Ack-1、CDC42在胃癌组织中的表达明显升高，其中CDC42和肿瘤的浸润深度有关。Ack-1和CDC42的表达呈明显正相关，提示二者在胃癌发生发展中起重要作用，有可能成为胃癌预后的判断指标和治疗靶点。

关键词Ack-1CDC42荧光定量PCR免疫印迹胃癌

中图分类号R735．2

文献标识码A

DOI：10.13452/j.cnki.jqmc.2015.03.008

AbstractObjectiveTo study the Ack-1and CDC42 expression in gastric cancer and its difference in the clinical and pathological features.Methods 42 cases of gastric cancer tissue confirmed by pathology and its adjacent normal tissue were collected.The expression of Ack-1 and CDC42 were evaluated by Real-time PCR and Western-blot.Results Both expression of Ack-1 and CDC42 in gastric cancer tissue were significantly increased compared with those in the adjacent normal tissue at the protein and mRNA level.The increased CDC42 was associated with local invasion in gastric carcinoma(P<0.01).And there was a positive correlation between the expression of CDC42 and Ack-1(r=0.481，P<0.01).Conclusion CDC42 and Ack-1 are obviously over expressed in gastric carcinoma tissue compared with those in the adjacent normal tissue at the protein and mRNA level，and there show a positive correlation between them.It is considered that they may play an important roles in the development and progression of gastric cancer，and may be a potential biomarkers for the prognosis and treatment of gastric cancer in the future.

KeywordsCDC42 Ack-1Real-timePCR Western-blotGastric cancer

收稿日期2015-02-04

EXPRESS AND SIGNIFICANCE OF CDC42 AND

Ack-1 IN GASTRIC CANCER

Gao Qian，Lu Yongfu，Wang Yun，Yang Yongcheng，Jiang Hanmei

(Department of Gastroenterology，The Affiliated Hospital of Qinghai University，Xining，Qinghai 810001，China )

胃癌发病机制尚有许多未明之处。近年来Rho家族蛋白在肿瘤方面的作用受到人们重视，其中CDC42是一个具有代表性的小分子G蛋白，它在细胞骨架调节、细胞运动、细胞凋亡、细胞恶性转化及肿瘤的浸润和转移等方面有重要作用[1-2]。Ack-1是CDC42下游的效应原件，主要分布于胞浆的非受体酪氨酸激酶，具有催化底物蛋白酪氨酸残基磷酸化激活底物酶活性的功能。近年来，国内外研究显示，Ack-1的表达、激活与肿瘤的发生侵袭转移等过程相关，但其在胃癌中的作用目前研究较少，且结论不一，Ack-1和CDC42在胃癌中的关系不明。本研究旨在通过实时定量PCR(real-time PCR)和蛋白免疫印迹(Western-blot)法检测Ack-1和CDC42在胃癌组织中的表达，探讨二者在胃癌组织中的表达特点及和胃癌临床病理特点的关系。……