*：教育部春晖计划项目(Z2012083)；国家自然科学基金资助项目(81360687)

三味檀香散预处理对低压低氧性大鼠右心室重构的影响*

陈宏1，赵媛2，李亚楠3，李永芳3，李瑞莲3，杨梅3**

(1.青海省妇女儿童医院；2.武警青海总队医院；3.青海大学医学院)

摘要目的研究三味檀香散对低压低氧性大鼠右心室重构的影响。方法将大鼠置于低压氧舱内(模拟海拔5000m高原)饲养4周，建立低压低氧性大鼠右心室重构的动物模型。实验大鼠随机分为正常组，模型组，三味檀香散大、中、小剂量组。分别测定各组大鼠平均肺动脉压(mPAP)、平均颈动脉压(mCAP) 和右室肥厚指数(RVHI)，并测定心肌匀浆中超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)活性和丙二醛(MDA)含量；取心肌组织，HE染色，光镜下观察形态学变化。结果模型组大鼠的mPAP和RVHI明显升高，SOD和GSH-Px活性降低，MDA含量升高，与正常组比较均有显著差异(P<0.01)；与模型组比较，三味檀香散预处理各剂量组大鼠的mPAP和RVHI明显降低(P<0.05或P<0.01)，SOD和GSH-Px活性明显升高(P<0.05或P<0.01)，三味檀香散大剂量组大鼠MDA降低(P<0.05)，而mCAP在各组大鼠之间无统计学差异。结论三味檀香散预处理能明显降低大鼠低氧所引起的肺动脉高压和右心室肥厚，减轻氧化应激损伤，对低氧性大鼠右心室重构有一定的保护作用。

关键词三味檀香散右心室重构低氧

通讯作者陈宏(1965～ )，女，汉族，青海籍，副主任医师. **：，教授，E-mail： ym_0208@163.com

中图分类号R965

文献标识码A

DOI：10.13452/j.cnki.jqmc.2015.03.003

AbstractObjectiveThe study was designed to observe the effect of Sanwei tanxiang powder on hypoxic right ventricular remodeling in rats.Methods The rats were kept in a hypobaric chamber which simulated the environment of a 5000 m altitude for four weeks，in order to establish hypoxic right ventricular remodeling model，The mean pulmonary arterial pressure(mPAP)and mean carotid arterial pressure(mCAP )were recorded，right ventricular hypertrophy index(RVHI)were calculated，the activity of SOD，GSH-Px and the level of MDA in heart homogenates of all groups were measured.The pathological changes were observed by photo-microscope for evaluating the effect.Results Compared with normal group，the mPAP and RVHI were higher，the activity of SOD，GSH-Px were decreased，the level of MDA was higher in model group(P<0.01).Compared with model group，the mPAP and RVHI in experimental groups were decreased significantly(P<0.05 or P<0.01)，the activity of SOD，GSH-Px were significantly higher in Sanwei tanxiang powder treated groups(P<0.05 or P<0.01).The MDA in Sanwei tanxiang powder high dose group was decreased(P<0.05).It obviously improved the development of ultra structural pathological changes in the rats treated with Sanwei tanxiang powder.The mCAP is no difference between all of the groups.Conclusions The Sanwei tanxiang powder has a protective effect against hypoxic right ventricular remodeling in rats by decreasing the mPAP and RVHI.

KeywordsSanwei tanxiang powderRight ventricular remodelingHypoxia

收稿日期2015-01-06

EFFECT OF SANWEI TANXIANG POWDER ON HYPOTENSIVE

AND HYPOXIC RIGHT VENTRICULAR REMODELING IN RATS

Chen Hong1，Zhao Yuan2，Li YaNan3，Li Yongfang3，Li Ruilian3，Yang Mei**3

(1.Qinghai Provincial Hospital of Women and Children；2.Armed police corps hospital in qinghai；

3.Qinghai University Medical College)

急性与慢性高原低氧对机体的影响及机体对低氧环境的适应机制深受人们关注。心血管系统是高原低氧环境下受影响较大的系统之一，高原低氧对心血管系统的影响：低氧引起肺动脉血管收缩、阻力增加，进而加重右心室后负荷，严重时导致右室重构、心力衰竭[1-2]，而右心室重构是导致高原心脏病心衰的主要原因。据流行病学调查， 高原心脏病发病率随着海拔的升高而增加，而且移居者明显高于世居者。……