研究报告

野生树鼩肠道蠕虫感染调查及分析

孙晓梅，仝品芬，匡德宣，陈玲霞，王文广，陆彩霞，代解杰

(中国医学科学院北京协和医学院医学生物学研究所树鼩种质资源中心，

云南省重大传染病疫苗研发重点实验室，昆明650118)

【摘要】目的调查野生树鼩(Tupaiabelangeri Chinensis) 感染肠道蠕虫的主要种类并进行鉴定，为今后树鼩寄生虫检测提供形态学参考，为实验树鼩寄生虫控制提供依据。方法采集203只野外来源的树鼩新鲜粪便，虫卵采用常规粪便直接涂片以及孵化后显微镜观察；绦虫采用压片、固定染色，以及线虫经透明后体视镜观察，虫卵与成虫相对应鉴定。结果野生树鼩肠道蠕虫的总感染率为75.86%，主要感染种类有3种，经鉴定为长膜壳绦虫、奇口线虫和粪类圆线虫，感染率分别为27.67%，30.06%和51.52%。三种蠕虫的混合感染率为4.55%。两种线虫虫卵在树鼩粪便中多为含胚胎形态。 结论 野生树鼩肠道蠕虫的感染率较高。对野外引入的新种源必须隔离检疫，进行针对性的药物治疗，才能有效地控制肠道寄生虫病的传播。

【关键词】树鼩；肠道蠕虫；长膜壳绦虫；奇口线虫；粪类圆线虫

[基金项目]国家科技支撑计划 (2011BAI15B01-21)，云南省科技创新平台建设(2013DA002)。

[作者简介]孙晓梅(1963-)，女，研究方向：实验动物病原学研究。E-mail： sxm@imbcams.com.cn。

[通讯作者]代解杰(1961-)，男，研究员。E-mail： djj@imbcams.com.cn。

【中图分类号】R33【文献标识码】 A

doi:10.3969.j.issn.1671.7856. 2015.002.004

Survey of intestinal helminth infection in wild tree shrews

(TupaiabelangeriChinensis)

SUN Xiao-mei, TONG Pin-fen, KUANG De-xuan, CHEN Ling-xia, WANG Wen-guang, LU Cai-xia, DAI Jie-jie

(Center of Tree shrews Germplasm Resources, Institute of Medical Biology, Chinese Academy of Medical Sciences and

Peking Union Medical College, Yunnan Key Laboratory of Vaccine Research & Development on Severe

Infection Diseases, Kunming 650118, China)

Abstract【】ObjectiveTo analyze and understand the epidemic situation of intestinal helminthe infection in wild tree shrews (Tupaia belangeri Chinensis), and to provide useful reference and support for prevention and treatment of parasitic infection in tree shrews. Methods 203 wild tree shrews were used in this study. Stool ova in the smear from fresh feces and after incubation were observed and identified by routine microscopic examination. Cestodes were observed in press slide after fixation and staining, and nematodes were observed under a stereoscope after clearing. The ova were identified with corresponding adult parasites. Results In this survey, we found the total prevalence rate of intestinal helminthes in this wild tree shrew population was 75.86%, mainly including three kinds of important parasites, namely, Hymenolepis diminuta 27.67%, Rictularia sp. 30.06%, and Strongyloides stercoralis 51.52%, respectively. Mixed infection rate of the three species was 4.55%. For Rictularia sp. and Strongyloides stercoralis, embryonated eggs were often observed in their fresh feces. ConclusionsIntestinal helminth infection rate is rather high in the wild tree shrews. Quarantine inspection is necessary for new intake. Different control measures and surveillance should be taken to control the dissemination of intestinal helminth infection in tree shrews.

【Key words】Tree shrews; Intestinal helminthes;Hymenolepisdiminuta;Rictulariasp.;Strongyloidesstercoralis

寄生虫感染不仅危害实验动物健康，对实验结果造成影响，甚至引起人兽共患疾病的传播，在使用实验动物开展生命科学研究过程中必须重视对实验动物寄生虫的控制。……