研究报告

姜黄素抑制人宫颈癌CaSki细胞裸鼠移植瘤的生长

刘冬菊1,姚宇1，黄文革2

(1． 广州中医药大学第三附属医院, 广州510360; 2. 中山大学实验动物中心，广州510080)

【摘要】目的探讨姜黄素对人宫颈癌CaSki细胞裸鼠皮下移植瘤生长的抑制作用。方法建立人宫颈癌裸鼠皮下移植瘤模型，35只荷瘤裸鼠随机分为5组，姜黄素高剂量(200 mg/kg)、中剂量(100 mg/kg)、低剂量(50 mg/kg)组、溶剂对照组和顺铂组(3 mg /kg)，每组7只，姜黄素连续灌胃给药15 d，顺铂组每3 d腹腔注射给药，共5次。停药24 h后处死裸鼠，测量裸鼠体重、移植瘤体积、瘤重，计算抑瘤率；光镜下观察移植瘤组织形态学变化；结果处理组的移植瘤体积均明显小于空白对照组(P

【关键词】宫颈癌；姜黄素；移植瘤

[基金项目]广东省科技计划

[通讯作者]刘冬菊(1971-)，女，副主任医师，硕士生导师，主要从事妇科肿瘤的研究。Email: weila_dongju@163.com。

【中图分类号】R33【文献标识码】 A

doi:10.3969.j.issn.1671.7856. 2015.002.002

Inhibitory effect of curcumin on the growth of human cervical

carcinoma (CaSki) xenografts in nude mice

LIU Dong-ju1，YAO Yu1，HUANG Wen-ge2

(1. the Third Affiliated Hospital of Guangzhou University of Traditional Chinese Medicine, Guangzhou 510360, China；

2. Laboratory Animal Center of Zhongshan University, Guangzhou 510080)

Abstract【】ObjectiveTo study the inhibitory effect of curcumin on the growth of human cervical carcinoma CaSki xenografts in nude mice. MethodsHuman cervical cancer cell line CaSki cells were inoculated subcutaneously into nude mice. Thirty-five tumor-bearing mice were randomly divided into 5 groups: curcumin high dose (200 mg/kg), moderate dose (100 mg/kg), low dose (50 mg/kg), solvent control group and cisplatin group (3 mg/kg), 7 mice in each group. Curcumin was orally administered once per day for consecutive 15 days. Intraperitoneal injection of cisplatin was given every three days, for a total of five times. The mice were sacrificed 24 h after the end of treatment and the body weight, tumor volume, tumor weight were measured and the inhibition rate was calculated. The histological changes of tumor tissue was examined. Results The tumor volumes of the treated group were significantly lower than that of the control group (P<0.01). The tumor inhibition rate of the curcumin high, medium and low dose groups were 44.3%, 40.1% and 33.3%, respectively. Apparent necrosis was observed in the tumors of the curcumin-treated groups. ConclusionCurcumin possesses apparent inhibitory effect on the growth of transplanted tumors of human cervical cancer CaSki cells in nude mice.

【Key words】Cervical cancer; Curcumin; Xenografts; Nude mice

宫颈癌是最常见的妇科恶性肿瘤，发病率居女性恶性肿瘤第二位。我国每年子宫颈癌新病例数为13.15万，约占全球总数的1/3，每年有2万～3万妇女死于子宫颈癌。以顺铂为基础的化放疗晚期及转移宫颈癌主要的治疗方式。顺铂严重的消化道反应、肾毒性、神经毒性及骨髓移植的不良反应使其在临床应用受到限制。因此迫切需要寻找并研究高效无毒副作用的新药。大量研究发现中药姜黄素(curcumin)具有多种生物学活性，是一种具有良好发展前景的药物。它具有抗癌广谱、不良反应小的优点，已成为目前研究热点。本文主要探讨姜黄素对人宫颈癌CaSki细胞裸鼠移植瘤生长的作用。……