研究报告

黄连解毒汤对小鼠酒精性脂肪肝的预防作用

王睿林1，李晓娟2，白云峰1，王立福1，李蓓2，李永纲1，李瑞生2

(1. 解放军第302医院中西医结合肝病诊疗与研究中心，北京100039；

2. 解放军第302医院实验技术研究保障中心，北京100039)

【摘要】目的研究黄连解毒汤(HLJDT)对小鼠酒精性肝损伤的预防作用，为黄连解毒汤临床防治酒精性肝病提供实验依据。方法选取60只C57小鼠随机分为空白对照组、模型对照组和HLJDT低、中、高剂量(0.61、1.22、2.44 g/kg/d)模型组，每组各12只。模型组用50%乙醇灌胃，同时采用HLJDT进行实验干预，6周后，对各组小鼠的体质量、肝指数、血清生化指标以及肝脏和结肠的病理学改变进行综合对比分析。结果HLJDT各剂量模型组小鼠的体重增长量均显著增加(P<0.05)，肝指数明显降低(P<0.05)，同时HLJDT各剂量模型组能显著降低血清ALT、AST和TG的含量(P<0.05)。病理结果显示HLJDT各剂量均对酒精性肝损伤有显著的改善作用，且从低剂量到高剂量组对肠粘膜通透性具有递增的改善作用。结论黄连解毒汤可显著降低肠粘膜的通透性，对小鼠酒精性肝损伤具有一定的预防保护作用。

【关键词】黄连解毒汤；酒精性脂肪肝；小鼠

[基金项目]解放军第302医院院长创新基金(YNKT2013026)。

[作者简介]王睿林(1979-)，男，博士，主治医师，从事中西医结合治疗研究，E-mail： wrl7905@163.com；李晓娟(1980-)，女，硕士，E-mail： sxlily55@163.com。并列为第一作者。

[通讯作者]李瑞生(1969-)，男，博士，副研究员，从事人类疾病动物模型研究，E-mail： lrsheng@sohu.com；李永纲(1965-)，男，硕士，主任医师，从事传染病学研究，E-mail： lyg100039@sina.com。

【中图分类号】R33【文献标识码】 A

doi:10.3969.j.issn.1671.7856. 2015.002.009

Preventive effects of Huanglian Jiedu Decoction on

alcoholic fatty liver in mice

WANG Rui-lin1，LI Xiao-juan2，BAI Yun-feng1，WANG Li-fu1，LI Bei2，LI Yong-gang1，LI Rui-sheng2

(1. Liver Disease Center for Combined TCM and Western Medicine，

2. Research and Technology Service Center，302 Hospital of PLA，Beijing 100039，China)

Abstract【】ObjectiveTo study the preventive effect of a Chinese traditional medicine, Huanglian Jiedu Decoction (HLJDT), on alcoholic fatty liver in mice, and provide experimental evidence for clinical prevention and treatment of alcoholic liver disease. Methods Sixty healthy SPF male C57 mice were randomly divided into control group, model group and low, medium and high dose HLJDT(0.61, 1.22, 2.44 g/kg/d)groups, 12 mice in each group. The mice of model groups were given gastric gavage of 50% ethanol, combined with concomitant HLJDT intervention for 6 weeks. The mice of control group received the same volume of saline. The body weight, liver index, serum biochemical indexes were determined and histopathological changes in the liver and colon tissues were examined and analyzed at 6 weeks after the experiment. ResultsBody weight gains of the mice in all the three dose HLJDT groups were significantly higher than that of the control group (P<0.05)，liver index and the serum ALT, AST and TG contents were significantly decreased (P<0.05 for all). Histological changes in the liver of all the three dose HLJDT groups were significantly milder than those of the model control group, and also improving of the colon mucosal alterations was observed in the three dose HLJDT groups in a dose-dependent manner. ConclusionsHuanglian Jiedu Decoction can well reduce the permeability of intestinal mucosa, and exerts certain preventive and protective effects on alcoholic liver injury in mice.

【Key words】Huanglian Jiedu decoction; Alcoholic fatty liver; Mice

酒精性肝病(alcoholic liver disease，ALD)是一种进行性发展且后期会严重危害身体健康甚至威胁生命的消化系统疾病。其致病因素单一而明确，主要是长期大量饮酒所造成的，但其发病机制却十分复杂[1]。近年来酒精致肠粘膜通透性增加，肠道内毒素渗漏入血及其激活肝Kupffer细胞产生炎症细胞因子机制，是酒精性肝损伤机理研究的热点之一[2,3]。而黄连解毒汤(HLJDT)是由黄连、黄柏、黄芩、桅子四味药组成的清热解毒的代表方，具有泻火解毒之功效，主治实热火毒，三焦热盛之证[4]。……