研究报告

四物汤干预减轻乙醇对小鼠肝脏的损伤作用

白云峰1，李晓娟2，李永纲1，王立福1，李蓓2，王睿林1，李瑞生2

(1. 解放军第302医院中西医结合肝病诊疗与研究中心，北京100039；

2. 解放军第302医院实验技术研究保障中心，北京100039)

【摘要】目的通过四物汤对小鼠酒精性肝损伤的干预效果的研究，阐明其对乙醇损伤肝脏的影响，为四物汤临床防治酒精性肝病提供实验数据。方法选择60只C57小鼠，随机分为对照组、模型组和四物汤低、中、高剂量(0.91、1.82、3.64 g/kg/d)组，每组12只，用50%乙醇灌胃建立酒精性脂肪肝模型，同时用四物汤进行干预。实验6周后，对各组小鼠的体质量、肝指数、血清生化指标和肝脏病理学改变进行综合对比分析。结果四物汤各剂量组小鼠与模型组相比其体重增长量均显著增加(P<0.05)，肝指数明显降低(P<0.05)，血清ALT、AST和TG含量(P<0.05)均显著降低。四物汤从低到高剂量组的肝脏外观从粗糙逐渐变光滑，颜色逐渐加深。病理结果也显示肝脏脂肪变随着剂量从低到高呈递减的程度，而高剂量组小鼠的脂肪变改善效果最显著。结论四物汤对小鼠酒精性肝损伤具有一定的保护作用。

【关键词】四物汤；乙醇； 肝脏损伤

[基金项目]解放军第302医院院长创新基金：YNKT2013026。

[作者简介]白云峰(1970-)，男，博士，E-mail： yunfengbai3@yahoo.com.cn；李晓娟(1980-)，女，硕士，E-mail： sxlily55@163.com。并列第一作者。

[通讯作者]李瑞生(1969-)，男，博士，副研究员，从事人类疾病动物模型研究，E-mail： lrsheng@sohu.com；王睿林(1979-)，男，博士，主治医师，从事中西医结合治疗研究，E-mail： wrl7905@163.com。

【中图分类号】R33【文献标识码】 A

doi:10.3969.j.issn.1671.7856. 2015.002.003

Protective effect of Siwu decoction on alcoholic hepatic injury in mice

BAI Yun-feng1，LI Xiao-juan2，LI Yong-gang1，WANG Li-fu1，LI Bei2，WANG Rui-lin1，LI Rui-sheng2

(1. Liver Disease Center of Combined TCM and Western Medicine，

2. Research and Technology Service Center, 302 Hospital of PLA, Beijing 100039，China)

Abstract【】ObjectiveTo provide experimental data for clinical prevention and treatment of alcoholic liver disease in mice by a Chinese medicine, Siwu decoction, and study the intervention effect of Siwu decoction on alcoholic liver injury. MethodsSixty C57 mice were selected and randomly divided into the control group，model group and Siwu decoction low, medium and high dose (0.91, 1.82, 3.64 g/kg/d) groups, 12 mice in each group. The mouse models of alcoholic fatty liver were generated with gastric gavage of 50% ethanol, and Siwu decoction intervention was applied to these mouse models. Six weeks later，the mice body weight，liver index，serum biochemical indexes and liver pathological changes were determined and analyzed. ResultsCompared with the model group, Body weight gains of mice in all dose Siwu Decoction groups were significantly increased (P<0.05)，and liver indexes significantly decreased (P<0.05). The serum levels of ALT, AST and TG content significantly decreased(P< 0.05)．The liver gross appearance was gradually changed from rough to smooth and the color gradually deepened in the mice of Siwu decoction groups, increasingly from low to high dose group．Pathological examination showed a decrease of hepatic steatosis in the mice treated with Siwu decoction from low dose to high dose, and mice in the high dose group showed the best improvement of hepatic steatosis. Conclusion The Chinese medicine Siwu decoction has a protective effect on alcoholic liver injury in mice.

【Key words】Siwu decoction; Ethanol; Liver damage

酒精性肝病(alcoholic liver disease, ALD)是由于长期大量饮酒所导致的肝脏疾病，由于嗜酒已成为当今世界日益严重的公共卫生问题，因此酒精性肝病的研究也成为全球的研究热点[1]。乙醇进入体内会经过多种途径对肝脏造成损伤[2]，其中由乙醇引起氧化应激所产生的自由基可使线粒体膜通透性增加[3]，引起细胞能量代谢障碍而导致肝细胞损伤。……