湖北地区禽源性空肠弯曲菌的分离鉴定及耐药性分析
2016-01-08董俊韩梅邵华斌罗青平刘国平张腾飞
董俊+韩梅+邵华斌+罗青平+刘国平+张腾飞


摘要:为了解湖北省禽源空肠弯曲菌的流行病学及耐药性情况,从武汉市及其周边地区采取禽源样品276份,其中肛拭子175份,盲肠内容物101份。共分离出50株空肠弯曲菌,分离率为18.12%,其中肛拭子分离20株,盲肠内容物分离30株。结果显示,不同采样途径空肠弯曲菌的分离率不同,盲肠内容物分离率高于肛拭子。采用定性测定纸片扩散法对以上分离的菌株进行药敏试验,50株空肠弯曲菌分离株对氨基糖苷类药物敏感性最高(S≥66.0%),其次分别是红霉素(S=70.0%)和磺胺甲基异恶唑(S=58.0%)。对喹诺酮类药物耐药性最强(R=100%)。
关键词:弯曲菌;分离鉴定;耐药性
中图分类号:S852.61+3 文献标识码:A 文章编号:0439-8114(2015)23-5959-04
DOI:10.14088/j.cnki.issn0439-8114.2015.23.042
Isolation,Identification and Antimicrobial Susceptibility Proflles of Campylobacter jejuni Isolates from Poultry in Hubei
DONG Jun1,2,HAN Mei2,SHAO Hua-bin2,LUO Qing-ping2,LIU Guo-ping1,ZHANG Teng-fei2
(1.College of Animal Science, Yangtze University, Jingzhou 434025, Hubei,China;
2.Institute of Animal Husbandry and Veterinary, Hubei Academy of Agricultural Sciences, Wuhan 430064,China)
Abstract: To investigate the epidemiological and antimicrobial susceptibility profiles of Campylobacter jejuni in poultry in Hubei province, 276 samples was collected, thereinto 175 were from feces and 101 were from contents of cecum. 50 (18.12%) Campylobacter jejuni was isolated in total, among the isolates, 20 isolates were separated from feces, and 30 isolates were separated from the contents of cecum. The results showed that the separation rate from contents of cecum was higher than that from feces. The disk diffusion test method was used to determine resistance of the isolates. The results showed that the isolates were most sensitive to aminoglycosides(S≥66%),secondly to erythromycin,sulfamethoxazole, and all strains were resistant to floroquinolones (R=100%).
Key words:Campylobacter jejuni;Isolation and Identification;Drug resistance
弯曲菌(Campylobacter)是自然界中广泛存在的、全球范围内主要的食源性人兽共患性病原菌之一[1]。对人致病的弯曲菌中 93.4%为空肠弯曲菌(Campylobacter jejuni,C. jejuni),2.3%为结肠弯曲菌(Campylobacter coli,C. coli),其他弯曲菌偶尔致病[2]。特定血清型空肠弯曲菌引起的肠炎是格林-巴利综合征(Guillain-Barre syndrome,GBS)的重要前驱因子[3-5]。
近年来,许多国家空肠弯曲菌发病率呈指数增长趋势,主要引起急性肠炎和食物中毒,并伴发反应性关节炎、肝炎、瑞特氏病和格林-巴利综合征等免疫性损伤性疾病。在兽医临床中,空肠弯曲菌可引起家畜流产、不孕、乳房炎及幼畜腹泻和家禽肝炎[6-9]。空肠弯曲菌是野生、家养动物的正常寄居菌,感染的动物通常无明显病症,但可长期向外界排菌,通过排泄物或分娩污染食物和饮水,从而引起人类感染。其中,家禽是空肠弯曲菌病最重要的传染源之一。……
