·中药药理·

桃红四物汤对兔皮片移植模型血管生成素影响的研究

黄承宇1，孙绍裘2*，李益亮2

（1.湖南中医药大学,湖南长沙410208;2.湖南中医药大学第二附属医院,湖南长沙410005）

〔摘要〕目的通过检测兔皮片移植后桃红四物汤对血管生成素（angiogenin,Ang）血清含量及皮片吻合区基因表达情况的影响，探讨桃红四物汤对皮片移植中促进血管新生的作用机制。方法将75只实验兔按随机数字表法将其分为五组，即桃红四物汤低、中、高剂量组、模型组和假手术组，每组15只。造模后分组干预，并于术后2、5、8d三个时限，从每组随机抽取5只实验兔，心脏采血后予以耳缘静脉注射空气处死，并切取移植皮片吻合区皮片组织。通过ELISA和RT-PCR检测血清样品中Ang的含量及皮片吻合区基因表达情况。结果桃红四物汤三组中移植皮片的新生血管密度明显高于模型组及假手术组（P＜0.05），差异有统计学意义。Ang血清含量及基因表达中桃红四物汤三组表达量高于模型组及假手术组（P＜0.05），差异有统计学意义。结论桃红四物汤能上调组织中血管新生促进因子Ang的表达，促进移植皮片成活。

〔关键词〕桃红四物汤;皮片移植;血管生成素;血管新生

Effects of Taohongsiwu Decoction on Angiogenin of Rabbit Skin Graft Models

HUANG Chengyu1, SUN Shaoqiu2*, LI Yiliang2

(1. Hunan University of Chinese Medicine, Changsha, Hunan 410208, China; 2. The Second Affiliated Hospital of Hunan University of Chinese Medicine, Changsha, Hunan 410005, China)

〔Abstract〕Objective To investigate the mechanism of Taohongsiwu Decoction on promoting angiogenesis skin grafby detectingserum angiogenin (Ang) concentration and gene expression. Methods 75rabbits were randomly divided into 5groups, namely, low dose Taohongsiwu Decoction group, middle dose Taohongsiwu Decoction group, high dose Taohongsiwu Decoction group, model group and sham operation group, 15rats in each group. After grouping intervention, at the three time-points: 2d, 5d, 8d, 5rabbits were randomly selected from each group.After blood collection, the rats were executed by air injection of the ear vein, and the skin grafts anastomosed area skin tissues were cut. The Ang content in serum samples and gene expression in anastomosed area were detected by ELISA and RT -PCR. Results The microvessel density of skin grafts in Taohongsiwu Decoction groups was significantly better than the model group and operation group (P<0.05), the difference was statistically significant. Ang content in serum samples and gene expression in the Taohongsiwu Decoction groups were higher than that of the model group and operation group(P<0.05), the difference was statistically significant. Conclusion Taohongsiwu Decoction can upregulate Ang expression in the tissues to promote the survival of skin graft.

〔Keywords〕Taohongsiwu Decoction; skin graft; angiogenin; angiogenesis

皮肤是人体的主要器官之一，覆盖着整个身体表面，不仅具有感觉、调节体温以及分泌排泄等功能，而且还能阻止病菌或其他有害物体的入侵，防止体液、电解质和蛋白质等损失，保护生命和维持机体与环境相适应。一旦皮肤发生缺损而不能及时获得修复，就会失去正常外形与功能活动，甚至危及生命。……