·针推经络·

针刺对脑缺血再灌注大鼠相关miRNA数目的影响

陈文，林亚平，杨茜芸，刘琴，肖姮，田浩梅*

（湖南中医药大学针灸推拿学院，湖南长沙410208）

〔摘要〕目的观察针刺疗法对脑缺血再灌注大鼠相关miRNA数目的影响。方法参照ZeaLonga线拴法复制大脑中动脉缺血模型(MCAO)并加以改良，将40只SD大鼠随机分为空白组、假手术组、模型组、针刺组，治疗72h后，用基因微阵列技术筛选差异表达miRNA，并对神经功能缺损评分、TTC染色检测的梗死面积比进行比较。结果与空白组及假手术组比较，模型组大鼠神经功能缺损评分，梗死面积比增多，差异有统计学意义(P＜0.05)；针刺治疗组相对应指标则不同程度地降低，差异有统计学意义(P＜0.05)；与空白组比较，模型组有4个差异基因表达；与假手术相比，模型组有5个差异基因表达；与模型组相比，针刺组有个16差异基因表达；差异均有统计学意义(P＜0.05)。结论针刺疗法可改善神经功能缺损，减少脑梗死面积及细胞凋亡，对脑组织起保护作用，其机制可能与针刺疗法调节相关的miRNA有关。

〔关键词〕针刺；脑缺血再灌注损伤；miRNA

Effects of Acupuncture on the Number of miRNA in Cerebral Ischemia Reperfusion Rats

CHEN Wen，LIN Yaping，YANG Qianyun, LIU Qin，XIAO Heng，TIAN Haomei*

(Acupuncture and Massage College of Hunan University of Chinese Medicine, Changsha, Hunan 410208, China)

〔Abstract〕Objective To observe the effect of acupuncture therapy on the number of miRNA in cerebral ischemia reperfusion rats. Methods Focal cerebral ischemia model was prepared with modified ZeaLonga' suture method. 40rats were randomly divided into 4groups，the blank group,the model group,the sham operation group,the acupuncture group,10in each group．After 72h treatment, the number of RNA express wasdetected with gene chip technology. The neurological function defect score and infarct size were compared. Results Compared with the blank group and sham operated group, the neurological deficit score and infarct area in the model group were significantly increased, the difference was statistically significant (P <0.05). The relative indexes of the acupuncture treatment group were decreased, and the difference was statistically significant (P<0.05). Compared with the blank group, there were 4differential gene expressions in the model group, compared with the sham operation, the model group had 5different gene expressions. Compared with the model group, the acupuncture group had a 16difference genes expression, the difference was statistically significant (P<0.05). Conclusion Acupuncture theapy can improve the neurological deficit, reduce the area of cerebral infarction and cell apoptosis, protect brain tissue, and its mechanism may be related to the regulation of related miRNA.

〔Keywords〕acupuncture; cerebral ischemia reperfusion injury; miRNA

脑缺血损伤主要有两类，缺血的原发性损伤与再灌注损伤。脑缺血再灌注损伤是指脑缺血致脑细胞损伤，恢复血液再灌注后，其缺血性损伤反而进一步加重的现象，是脑血管疾病发病的主要部分，且有高发病率、高致残率和高死亡率的特点。其过程包含一系列复杂的病理生理变化，机制极其复杂[1-2]。缺血期发生的病理生理变化并不是完全定型和不可逆的，血液恢复灌流后形成内源性损伤因子，启动一系列的病理生理过程导致脑缺血损伤进一步加重，即存在“缺血再灌注损伤”机制[3]，这种损伤是脑血管疾病发病的主要原因。……