白术七物颗粒对气阴两虚型STC大鼠结肠组织NSE的影响

谭蕊1，林仁敬2*，何永恒2

（1.湖南中医药大学，湖南长沙410208，2.湖南中医药大学第二附属医院，湖南长沙410005）

〔摘要〕目的研究白术七物颗粒对气阴两虚型结肠慢传输型便秘大鼠结肠组织神经元特异性烯醇化酶（neuron specific enolase，NSE）的影响，初步探讨白术七物颗粒对结肠慢传输型便秘的作用机制。方法将60只SD大鼠按照完全随机原则分为正常组、模型组、治疗组，每组各20只。正常组予以皮下注射等体积等渗生理盐水，模型组、治疗组予以皮下注射盐酸吗啡注射液2.5mg/(kg·d)，共45d，并同时灌服青皮（18g）、枳壳（18g）、附子（15g）溶液，建立气阴两虚型结肠慢传输型便秘模型。造模成功后各组分别予以相应药物进行灌胃治疗，每天1次，连续14d，末次给药后检测各组大鼠结肠组织中NSE的含量。结果造模前,各组大鼠粪便干重比较,差异无统计学意义；造模后，模型组、治疗组大鼠粪便干重与正常组比较明显减少，差异有统计学意义（P<0.01）；造模后,模型组、治疗组肠道炭末推进率与正常组比较明显降低，差异有统计学意义(P<0.01);模型组与治疗组大鼠结肠组织中NSE含量明显降低，与正常组相比，差异有统计学意义(P<0.05)；治疗组与模型组相比，NSE含量相对升高，差异有统计学意义(P<0.05)。结论白术七物颗粒能有效治疗气阴两虚型结肠慢传输型便秘，其作用与提升NSE的含量关系密切。

〔关键词〕结肠慢传输型便秘；白术七物颗粒；气阴两虚型；血管活性肠肽；NSE

Effects of BaizhuQiwu Granules on the NSE in Colon Tissue of STC Rats with Deficiency of both Qi and Yin Syndrome

TAN Rui1, LIN Renjing2*, HE Yongheng2

(1. Hunan University of Chinese Medicine, Changsha, Hunan 410208, China; 2. The Second Affiliated Hospital of Hunan University of Chinese Medicine, Changsha, Hunan 410005, China)

〔Abstract〕Objective To study the effect of Baizhu Qiwu Granules on neuron specific enolase (NSE) in colonic tissues of colon slow transit constipation rats with deficiency of both Qi and Yin syndrome and to explore its mechanism of action. Methods 60SD rats were randomly divided into normal group, model group and treatment group, 20rats in each group. The rats in normal group were given isovolumetric and isotonic normal saline in subcutaneous injections and the remaining rats in model and treatment groups were daily subcutaneous injection of 2.5mg/kg·d of morphine hydrochloride for total 45days, and at the same time administered with Qingpi (18g), Zhike (18g) and Fuzi (15g) to build thecolon slow transit constipation model rats with deficiency of both Qi and Yin syndrome.After establishing the models successfully, the rats in each group are given the corresponding drug treatment once daily for 14days. At the final injection,the level of NSE in colonic tissues of rats was measured. Results There was no significant difference in the dry weight of the feces of the rats in each group before building. After modeling, the dry weight of the feces of rats in the model group and the treatment group were significantly decreased compared with the normal group, the differences have statistical significance (P<0.01). The rate of intestinal charcoal propulsion in the model group and the treatment group were significantly decreased compared with the normal group, the differences have statistical significance (P<0.01). The content level of NSE in colon tissue of rats inthe model group and treatment group was increased compared to the normal group, the differences have statistical significance (P <0.05). The content level of NSE in colon tissue of rats in the treatment group was decreased compared to the normal group, the differences have statistical significance (P<0.05). Conclusion Baizhu Qiwu Granules can effectively treat both Qi and Yin type of colonic slow transit constipation,which may be related with the elevated NSE level.

〔Keywords〕slow transit constipation; Baizhu Qiwu Granules; deficiency of both Qi and Yin type; vasoactive intestinal peptide；NSE

结肠慢传输型便秘( slow transit constipation，STC)是一类主要以胃肠动力明显减弱为特点的顽固性便秘，迄今为止其病因仍不完全明确[1]。临床上常以便次减少、无便意、排便困难、排便不尽感为主要表现，症状顽固，治疗困难。目前其发病率有呈逐年上升的趋势，且有年轻化的趋向。本研究主要通过观察白术七物颗粒对气阴两虚型STC大鼠结肠组织神经元特异性烯醇化酶的含量影响，初步探讨白术七物颗粒对STC的治疗作用及其作用机制，为临床应用白术七物颗粒治疗本病提供初步实验依据。……