王哲，路芳，陈佃红，张平

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可逆性输卵管节育器干预大鼠子宫内膜异位症的实验研究

王哲，路芳，陈佃红，张平

目的：逆流法建立大鼠子宫内膜异位症（EMs）模型，观察可逆性输卵管节育器对大鼠EMs的预防作用。方法：30只大鼠随机分为3组，每组各10只，实验组逆流造模同时放置可逆性输卵管节育器，阴性对照组逆流造模，空白对照组不做处理。术后8周检测血清中抗子宫内膜抗体（EmAb）和CA125，观察组织形态学变化。结果：实验8周后3组大鼠血EmAb、CA125浓度差异有统计学意义（均P＜0.05）。阴性对照组血EmAb、CA125浓度高于空白对照组和实验组（均P＜0.05）。空白对照组无1例发病，阴性对照组8例发病，异位病灶体积（74.20±6.32）mm3，实验组1例发病，异位病灶体积为1.50mm3。结论：可逆性输卵管节育器可预防大鼠EMs的发生。

子宫内膜异位症；避孕器械；输卵管；动物实验；大鼠，Wistar；CA-125抗原Corresponding author：WANG Zhe，E-mail：jnwz63_@126.com；ZHANG Ping，E-mail:761298071@qq.com

【Abstract】Objective:To build the rat model of endometriosis by reflux method，observed the preventive effect of reversible oviduct synthetic on endometriosis in rats.Methods:The rats were divided into three groups randomly，experimental group（molding by reflux method and placement reversible oviduct synthetic），negative control group（molding by reflux method），blank control group（no processing）.Then tested EmAb and CA125 in serum，and observed the tissue morphological variations in eight weeks after operation.Results:There were difference in EmAb and CA125 in serum between three group（P＜0.05），and the negative control group was significantly higher than the experimental group and blank control group（P＜0.05）.There were also no obvious difference in histomorphology between the experimental group and the blank group.Neither of them developed endometriosis.In histomorphology observation，there is none in blank control group，8 cases in negative control group，ectopic lesions volume（74.20±6.30）mm3，1 case in experimental group，volume for the onset of ectopic lesions 1.50mm3.Conclusions: The reversible oviduct synthetic could prevent endometriosis in rats.

【Keywords】Endometriosis；Contraceptive devices；Fallopian tubes；Animal experimentation；Rats，Wistar；CA-125 antigen

（J Int Obstet Gynecol，2015，42：54-56）

子宫内膜异位症（EMs）是常见多发的妇科良性疾病，以痛经、不孕为主要表现，具有周期性发作、进行性加重、久治不愈等特点，是妇科疑难病症。该病多发生于生育期妇女，发病率呈逐年上升趋势，严重影响妇女的身心及生殖健康，目前尚无有效的预防方法。EMs发病机制复杂多样而又深奥，确切病因不明，既需宏观综合又需细致深入研究，目前较公认的学说为经血逆流学说。临床中常有引起子宫内膜逆流的操作，如液体膨宫下子宫内膜电切、纵隔切除、内膜息肉摘除、异物取出、输卵管插管等宫腔操作，腹腔镜下输卵管通液等，是否因此导致盆腔EMs有待商榷。本……