梁可等

[摘要] 目的 考察黄芪皂苷对化疗贫血模型小鼠血清中白介素-2（IL-2）、白介素-4（IL-4）和白介素-6（IL-6）的影响。 方法 选取6～7周龄昆明种小鼠64只，随机分为空白组、模型组、阳性对照组和给药组，每组16只，雌雄各半。采用环磷酰胺建立化疗贫血模型，空白组正常饲养，不予特殊处理。模型组、阳性对照组和给药组分别于1、3、5 d按0.2 g/kg体重剂量腹腔注射环磷酰胺。于造模第7天灌胃给药，空白组、模型组均按10 mL/kg体重胃饲蒸馏水；阳性对照组按5 mL/kg体重胃饲黄芪精混悬液；给药组按10 mL/kg体重胃饲黄芪皂苷溶液，灌胃治疗14 d后，摘眼球取血。利用酶联免疫吸附测定法（ELISA）测定血清中IL-2、IL-4和IL-6的含量。 结果 与空白组IL-2[（911.5±157.6）ng/L]、IL-4[（711.4±119.8）ng/L]、IL-6[（193.3±71.0）ng/L]含量比较，模型组IL-2[（245.0±104.3）ng/L]、IL-4[（124.3±54.9）ng/L]含量明显下降，IL-6[（1245.7±254.5）ng/L]含量显著升高，差异有统计学意义（P < 0.05）；与模型组比较，给药组IL-2[（640.8±192.8）ng/L]、IL-4[（574.0±112.4）ng/L]含量明显升高，IL-6[（521.5±203.8）ng/L]含量明显降低，差异有统计学意义（P < 0.05）。 结论 黄芪皂苷能提高化疗贫血模型小鼠血清中IL-2、IL-4的含量，降低IL-6的含量。IL-2、IL-4、IL-6与环磷酰胺引起的免疫失调和造血功能抑制有关；黄芪皂苷能够改善环磷酰胺所致的小鼠免疫力低下和骨髓抑制情况。

[关键词] 黄芪皂苷；化疗贫血；白介素-2；白介素-4；白介素-6

[中图分类号] R285.5 [文献标识码] A [文章编号] 1673-7210（2015）06（c）-0016-03

Effect of astragaloside on interleukin-2， interleukin-4 and interleukin-6 in chemotherapy induced anemic mice

LIANG Ke1 MA Jin2 QU Yi1 ZHENG Bingyuan1 QIAO Tie1

1.Department of Prescription， Liaoning University of Traditional Chinese Medicine， Liaoning Province， Shenyang 110847， China； 2.The Second Hospital Affiliated to Liaoning University of Traditional Chinese Medicine， Liaoning Province， Shenyang 110034， China

[Abstract] Objective To observe the effect of astragaloside on interleukin-2 （IL-2）， interleukin-4 （IL-4） and interleukin-6 （IL-6） in chemotherapy induced anemic mice. Methods 64 Kunming mice which were 6-7 weeks old were picked as the research objects and randomly divided into four groups： normal group， model group， positive control group and treated group. Each group was 16 mice， half male and half female. Chemotherapy anemia models were established by Cyclophosphamide. Normal group was fed without special treatment. Model group， positive control group and treated group were given 0.2 g/kg of Cyclophosphamide by intraperitoneal injection in 1， 3， 5 d， respectively. In 7 d， normal group and model group received 10 mL/kg of distilled water by intragastric administration； positive control group was fed 5 mL/kg of Huangqijing suspension； treated group was given 10 mL/kg of astragaloside solution. The mice were drawn blood from eyeball after 14 days treatment. The ELISA was used to test the concentrations of IL-2， IL-4 and IL-6 in mouse serum. Results Compared with the contents of IL-2 [（911.5±157.6） ng/L]， IL-4 [（711.4±119.8） ng/L] and IL-6 [（193.3±71.0） ng/L] of normal group， the concentrations of IL-2 [（245.0±104.3） ng/L]， IL-4 [（124.3±54.9） ng/L] in model group were significantly lower， and the concentration of IL-6 [（1245.7±254.5） ng/L] was evidently higher， the differences were statistically significant （P < 0.05）. Compared with model group， the contents of IL-2 [（640.8±192.8） ng/L]， IL-4 [（574.0±112.4） ng/L] were increased significantly， and the content of IL-6 [（521.5±203.8） ng/L] in treated group was obviously decreased， with statistically significant differences （P < 0.05）. Conclusion Astragaloside can increase the concentrations of IL-2 and IL-4， decrease the content of IL-6 in anemia model mice induced by chemotherapy. The changes in the contents of IL-2， IL-4 and IL-6 are associated with immune disorders and hematopoietic depression caused by Cyclophosphamide-induced anemia. Astragaloside can improve mouse low immunity and myelosuppression induced by Cyclophosphamide.

[Key words] Astragaloside； Chemotherapy-induced anemia； Interleukin-2； Interleukin-4； Interleukin-6

黄芪为豆科植物蒙古黄芪Astragalus membranaceus（Fisch.）Bge.var.mongholicus（Bge.）Hsiao或膜荚黄芪Astragalus membranaceus（Fisch.）Bge.的干燥根，具有补气升阳、固表止汗、利水消肿、生津养血之功效，能增强人体免疫系统功能，改善机体骨髓造血功能[1]。黄芪中皂苷类、黄酮类、多糖类为黄芪主要活性成分，而对其改善免疫和骨髓抑制作用，研究多集中于黄芪多糖，对黄芪皂苷的报道较少[2-3]。本实验主要通过建立小鼠化疗贫血模型，考察黄芪皂苷对小鼠血清细胞因子白介素-2（IL-2）、白介素-4（IL-4）、白介素-6（IL-6）的影响，从而对黄芪皂苷调节免疫功能、改善造血功能进行初步研究，为临床应用中药减轻化疗药毒副作用提供参考。

1 材料与方法

1.1 实验动物

SPF级昆明种小鼠64只，6～7周龄，雌雄各半，平均体重（20±2）g，购于辽宁中医药大学实验动物中心。……