陈英红等

[摘要] 目的 研究胆囊炎合剂与其功能主治相关的主要药效学。 方法 采用石胆酸引起的豚鼠急性胆囊炎模型观察胆囊炎合剂对胆囊炎的作用；采用四氯化碳及α-萘异硫氰酸酯所致动物急性肝损伤及黄疸型肝损伤模型观察胆囊炎合剂对肝损伤的保护作用；采用二甲苯引起的小鼠耳水肿和酵母所致大鼠发热方法观察其消炎解热作用。 结果 与石胆酸所致的急性胆囊炎模型组比较，豚鼠的肝脏、脾脏指数明显降低（P < 0.05或P < 0.01）；与四氯化碳所致急性肝损伤模型组和α-萘异硫氰酸酯致黄疸型肝损伤模型组比较，血清谷丙转氨酶、谷草转氨酶、总胆红素活性显著降低（P < 0.05）；胆囊炎合剂对二甲苯引起的炎症及酵母导致的发热也有显著的抑制作用（P < 0.05或P < 0.01）。 结论 胆囊炎合剂具有利胆、消炎、退黄及解热作用。

[关键词] 胆囊炎合剂；急性胆囊炎；肝损伤；药效学

[中图分类号] R285.5 [文献标识码] A [文章编号] 1673-7210（2015）06（c）-0008-05

Study on main pharmacodynamics of Cholecystitis Mixture

CHEN Yinghong1 XU Hong1 DONG Fang1 LUO Haoming2 LIU Wei1 JIANG Xiangzhi3▲

1.Institute of Chinese Materia Medica， Jilin Academy of Chinese Medical Sciences， Jilin Province， Changchun 130012， China； 2.College of Pharmacy， Changchun University of Chinese Medicine， Jilin Province， Changchun 130117， China； 3.Department of Pharmacy， Japan-China Union Hospital of Jilin University， Jilin Province， Changchun 130021， China

[Abstract] Objective To study the main pharmacodynamics of Cholecystitis Mixture related to its major functions. Methods The model of guinea pigs with acute cholecystitis caused by lithocholic acid was used to observe the function of Cholecystitis Mixture for cholecystitis； the models of animals with acute liver injury and liver injury of jaundice type caused by carbon tetrachloride and α-naphthylisothiocyanate were used to observe the protective effect of Cholecystitis Mixture for liver injury； the methods of ear edema of mice caused by xylene and fever of rats caused by yeast were used to observe the anti-inflammatory and antipyretic functions. Results Compared with model of acute cholecystitis caused by lithocholic acid， the liver and spleen indexes of guinea pigs were decreased significantly （P < 0.05 or P < 0.01）； compared with the model of acute liver injury caused by carbon tetrachloride and the model of liver injury of jaundice type caused by α-naphthylisothiocyanate， the activity of alanine transaminase， glutamic oxalacetic transaminase， total bilirubin was decreased significantly （P < 0.05）； Cholecystitis Mixture had marked inhibition effects for inflammations caused by xylene and fever caused by yeast （P < 0.05 or P < 0.01）. Conclusion Cholecystitis Mixture has the effects of choleretic effect， anti-inflammation， removing jaundice and relieving fever.

[Key words] Cholecystitis Mixture； Acute cholecystitis； Liver injury； Pharmacodynamics

按照吉林省对口援疆规划的总体安排和部署，为了充分发挥新疆阿勒泰地区珍贵药材的资源优势，哈医药在疾病治疗、预防保健等方面的传统医药优势，对目前临床使用安全可靠、疗效确切、用量较大的处方做出筛选，帮助新疆人民研究开发哈医药制剂，实现资源共享、互惠互利、共同发展的目标。胆囊炎合剂由一枝蒿、茵陈、大黄、金丝桃、菊苣和绵马贯众六味药制备而成。本方为新疆哈萨克名老中医经验方，临床应用多年，疗效显著。现作为院内制剂进行开发，根据其功能与主治对其进行了药效学的系统研究实验，现将结果报道如下：

1 材料与方法

1.1 试药

胆囊炎合剂由新疆阿勒泰地区哈萨克医院提供，为棕褐色液体，规格：每瓶100 mL，批号：20131204，临床用量：口服，25 mL/次，2次/d。消炎利胆片由广州白云山和记黄埔中药有限公司生产，规格：薄膜衣小片（0.26 g，相当于饮片2.6 g），产品批号：G2A009，功能主治：清热、祛湿、利胆，用于肝胆湿热所致的胁痛、口苦、急性胆囊炎、胆管炎见上述证候者，用法用量：口服，6片/次，3次/d。……