张宪亮+徐波+陆乐+何标+余锋+季浏

摘 要：为了解自主跑轮运动对C57BL/6小鼠空间学习记忆能力及海马内Notch信号通路的影响，选用12只雄性5周龄C57BL/6小鼠，随机分为运动组（R组，n=6只）和对照组（C组，n=6只），运动组进行8周的自主跑轮运动，对照组安静饲养，第8周开始利用Morris水迷宫测试其学习记忆能力。运动结束后，断颈处死小鼠取双侧海马，用Western Blot检测小鼠大脑海马内NICD的蛋白表达水平，用RT-PCR检测小鼠大脑海马内的Jagged-1、Notch-1、PS-1、Hes-1的mRNA表达水平。结果显示：Morris水迷宫实验显示，运动组小鼠学习记忆能力明显强于对照组;Western Blot测试，运动组小鼠海马内NICD表达显著上调，RT-PCR测试，运动组小鼠海马内Jagged-1 mRNA、Notch-1 mRNA、PS-1 mRNA和Hes-1 mRNA表达水平较对照组显著上调。结果说明，8周自主跑轮运动激活Notch信号通路，提高了海马空间学习记忆能力。

关 键 词：运动生物化学;学习记忆;Notch信号通路;自主跑轮运动;小鼠

中图分类号：G804.7 文献标志码：A 文章编号：1006-7116（2014）06-0134-05

Effects of voluntary wheel exercise on spatial learning and memory and

Notch signal pathway of mice

ZHANG Xian-liang1，XU Bo1，LU Le2，HE Biao1，YU Feng1，JI Liu1

（1.Key Laboratory of Adolescent Health Assessment and Exercise Intervention Ministry of Education，

School of Physical Education and Health，East China Normal University，Shanghai 200241，China;

2.School of Physical Education，Shanghai Normal University，Shanghai 200234，China）

Abstract： In order to understand the effects of voluntary wheel exercise on spatial learning and memory and Notch signal pathway in hippocampus of C57BL/6 mice， the authors prepared 12 5-week old male C57BL/6 mice， randomly divided them into an exercise group （group R， n=6） and a control group （group C， n=6）， let the mice in group R do a voluntary wheel exercise for 8 weeks， let the mice in group C live in a calm condition， starting from week 8， tested their learning and memory ability by utilizing Morris water maze， after the exercise was finished， cut the neck of the mice and took out hippocampus at both sides， measured the protein expression level of NICD in hippocampus of the mice by means of Western blot， measured the mRNA expression levels of Jagged-1， Notch-1， PS-1 and Hes-1 in hippocampus in the brains of the mice by means of RT-PCR， and revealed the following findings： the Morris water maze experiment showed that the learning and memory ability of the mice in group R was significantly stronger than that of the mice in group C; in Western blot test， the expression of NICD in hippocampus of the mice in group R increased significantly; in RT-PCR test， the mRNA expression levels of Jagged-1， Notch-1， PS-1 and Hes-1 in hippocampus of the mice in group R increased significantly as compared with those of the mice in group C. The said findings indicated that 8-week voluntary wheel exercise activated Notch signal pathway， enhanced the spatial learning and memory ability of hippocampus.endprint

Key words： sports biochemistry;learning and memory;Notch signal pathway;voluntary wheel exercise;mouse

运动作为中枢神经系统一种有效的刺激形式，在大脑结构和功能可塑性的调节中起重要作用，大量研究发现适宜的运动可以提高大脑学习记忆能力，但其机制是多方面的，也是不确切的。近来有研究发现，Notch信号通路在学习记忆能力的调节中起了关键作用[1]。Notch信号通路是一条传递细胞与细胞间信号的信号转导途径，在进化上高度保守，它精确调控着神经系统的发育分化及凋亡，并与突触可塑性、海马学习记忆能力等脑的高级功能密切相关。Ge等[2]发现敲除果蝇内的Notch基因导致其长期记忆能力下降，而过表达Notch基因野生型果蝇内可增强其长期记忆能力，提示Notch信号通路在果蝇的长期记忆能力中起了关键作用。……