姬立芹 陈文萍 范文勇 李大志

[摘要] 目的 探讨STOX1在子痫前期发病中的作用及临床意义。 方法 免疫组化检测正常妊娠及子痫前期胎盘组织中STOX1蛋白的表达情况；Western blot检测胎盘组织中STOX1蛋白的表达水平。 结果 STOX1蛋白主要表达于绒毛的合体滋养细胞和细胞滋养细胞，与正常妊娠组相比，子痫前期胎盘组织中STOX1蛋白的表达水平明显升高（P＜0.05），重度子痫前期组胎盘组织中STOX1蛋白的表达明显高于轻度子痫前期组（P＜0.05），轻度子痫前期组胎盘组织中STOX1蛋白的表达明显高于正常妊娠组（P＜0.05）。 结论 STOX1与子痫前期的发病密切相关，在子痫前期的发生、发展中可能起到重要作用。

[关键词] STOX1；子痫前期；胎盘

[中图分类号] R714.56???[文献标识码] A???[文章编号] 2095-0616（2012）15-31-03

Expression and significance of STOX1 in placenta of preeclampsia

JI?Liqin??CHEN?Wenping??FAN?Wenyong??LI?Dazhi

Department of Obstetrics and Gynecology,People's Hospital of Bao'an District in Shenzhen,Shenzhen 518101, China

[Abstract] Objective To investigate the expression of STOX1 protein in placenta of preeclampsia. MethodsImmunohistochemistry staining assay and western-blot was used to observe the expression of STOX1 protein in placental tissue from 67 cases of preeclampsia and35 cases of nomal pregnancy. Results STOX1 protein were mostly found in syncytiotrophoblast and cytotrophoblast.The expression level of STOX1 protein in preeclampsia placenta was significantly higher compared with the normal pregnancy group(P＜0.05).The expression level of STOX1 protein in group of sever preeclampsia was significantly higher compared with the mild pre-eclampsia group(P＜0.05),the expression level of STOX1 protein in group of mild preeclampsia was significantly higher than that in the group of normal pregnany(P＜0.05). Conclusion STOX1 may have a positive relationship with the degree of hypertensive disorder complicating pregnancy and play an important role in the pathogenesis and development of this disease.

[Key words] STOX1;Preeclampsia;Placenta

子痫前期是妊娠期高血压疾病的常见类型，严重影响母婴健康，病因及发病机制迄今不明，为研究的重点。子宫-胎盘缺血被认为是发病的重要原因之一，也是重要的病理改变。目前认为子宫-胎盘循环的有效建立与滋养细胞能否正常浸润胎盘床有关。STOX1是新发现的与子痫前期密切相关的母系印迹基因。研究表明，其在胎盘的生长发育中扮演着重要角色[1]。本研究通过免疫组化和Western blot技术检测子痫前期及正常妊娠组织中STOX1蛋白的表达情况，为进一步阐明子痫前期的发病机制提供理论基础。

1?资料与方法

1.1?一般资料

选取2010年10月～2011年10月笔者所在医院住院分娩的67例子痫前期患者作为观察组，同期住院分娩患者3 200例中随机抽取35例血压正常且蛋白尿阴性的患者作为对照组。……