刘志红 冯芳 王钢

【摘要】目的 研究高压氧对健康大鼠以及全身炎症反应综合征（SIRS）大鼠T淋巴细胞和细胞因子（IL-6、IL-10、TNF-α）等的影响。方法 40只雄性SD大鼠随机（随机数字法）分为5组。A组：腹腔注射生理盐水（0.9% NaCl, 5 ml/kg）作为对照组；B组：腹腔注射等量生理盐水，做3次高压氧治疗；C组：腹腔注射酵母多糖-石蜡悬液（500 mg/kg）；D组：腹腔注射等量酵母多糖-石蜡悬液，做1次高压氧治疗；E组：腹腔注射等量酵母多糖-石蜡悬液，做3次高压氧治疗。所有大鼠均于腹腔注射24 h后处死，门静脉取血，标本采用流式细胞技术对CD3⁺CD4⁺CD8⁺进行计数，应用ELISA方法检测IL-6、IL-10、TNF-α血浆水平，以单因素方差分析及LSD-t检验分析数据。结果 高压氧治疗可显著降低健康大鼠外周血CD4 T细胞百分比（P＜0.01），使CD4/CD8T细胞比值下降(P＜0.05)。酵母多糖腹腔注射使大鼠外周血CD4、CD8 T细胞均减少（P＜0.01）。1次和3次HBO治疗可使酵母多糖所致CD4 T细胞减少明显恢复(P＜0.01，P＜0.05)。对于未出现高炎症反应的大鼠，HBO治疗可增加IL-6（P＜0.05)和TNF-α（P＜0.01)，而减少IL-10（P＜0.05)。结论 高压氧可降低健康大鼠外周血CD4T细胞百分比。当出现高炎症反应时，高压氧可以明显抑制细胞因子TNF-α、IL-6和IL-10的爆发增多。高压氧对健康大鼠和未出现高炎症反应的SIRS大鼠起促炎作用。

【关键词】高压氧；SIRS；T淋巴细胞；细胞因子；免疫功能；TNF-α

Effect of hyperbaric oxygen on T-lymphoid cells and cytokines in rats LIU Zhi-hong, FENG Fang, WANG Gang. Emergency Department, First Affiliated Hospital, China Medical University, Shenyang 110001, China

Corresponding author: WANG Gang,Email:wg5385@sohu.com

【Abstract】Objective To investigate the effects of hyperbaric oxygen therapy on T-lymphocytes l and cytokines including IL-6, IL-10 and TNF-α as indicators well representing immune function and inflammation in normal rats and rats with systemic inflammatory response syndrome. Methods Forty Sprague-Dawley male Rats were randomly (random number) divided into five groups. Rats of group A were treated by saline solution（0.9℅ NaCl, 5 ml/kg）, and served as control group; rats of group B were treated with saline solution and hyperbaric oxygen therapy three times; rats of group C were treated with zymosan (500 mg/kg); rats of group D were treated with zymosan and hyperbaric oxygen therapy once; and rats of group E were treated with zymosan and hyperbaric oxygen therapy three times. At 24 hours after saline or zymosan administration, rats were sacrificed and blood samples were collected from the portal vein. CD3⁺,CD4⁺,CD8⁺cells were counted by flow cytometer, and plasma levels of IL-6, IL-10 and TNF-α were measured by ELISA . The results were analyzed by one-way ANOVA and LSD post-hoc test. Results CD4 T cell of normal rats was decreased by hyperbaric oxygen (P<0.01), CD4/CD8 T cell ratio was also decreased (P<0.05). Of rats with zymosan administration, CD4 T cells and CD8 T cells were both decreased on a parallel (P<0.01). A single one HBO and HBO for three times both raised the percentage of CD4 T cells which was significantly decreased by zymosan administration (P<0.01 and P<0.05). In rats without hyper-inflammation, hyperbaric oxygen therapy showed a pro-inflammatory effect by elevating TNF-α (P<0.01), IL-6(P<0.05) and reducing IL-10 (P<0.05). Conclusions HBO reduces CD4 T cells in normal rats. HBO reduces the production of TNF-α,IL-6 and IL-10 during hyper-inflammation. HBO is pro-inflammatory in normal rats and minor SIRS rats withourt appreciable hyper-inflammation in circulation.

【Key words】Hyperbaric oxygen;Systematic inflammatory response syndrome;T- lymphocyte; Cytokine; Immune function ;TNF-α

高压氧(HBO)作为一种治疗手段，广泛应用于临床。它可以促进受伤部位纤维母细胞、上皮细胞和血管的增值，增强白细胞的氧化杀伤能力，对特殊厌氧菌有致命的杀伤力^［1］。高压氧还表现出广泛的细胞分子学反应，包括细胞内信号转导和基因功能调节，高压氧的生化作用包括调节黏附分子、生长因子和细胞因子的表达，微循环的灌注和细胞能量的干预等^［2］。……