徐学娟 马学良 郭洪飞

[摘要] 目的 探讨复方甘草酸苷联合高频电刀对女性尖锐湿疣患者Th1/Th2 细胞因子的影响及疗效。 方法女性尖锐湿疣患者70例，随机分为两组：复方甘草酸苷联合高频电刀治疗1组；单纯高频电刀治疗2组。治疗前及治疗后1个月分别用ELISA 法检测各组患者外周血细胞因子IL—2、IL—4、IL—10及IFN—γ的表达水平。另设健康女性对照组25例，检测项目同上。 结果 ①复方甘草酸苷联合高频电刀治疗1组疗效明显高于单纯高频电刀治疗2组（χ2 =5.29， P < 0.05）；②女性尖锐湿疣患者与健康女性外周血Th1/Th2 细胞因子的表达比较：女性尖锐湿疣患者外周血中Th1型细胞因子IL—2（t = 4.012， P < 0.05）和IFN—γ（t=3.880， P < 0.05）的表达水平显著降低；而Th2型细胞因子IL—4（t = 2.694， P < 0.05）和IL—10（t = 3.069， P < .05）的表达水平显著升高；③治疗1个月后复方甘草酸苷联合高频电刀治疗1组相比单纯高频电刀治疗2组对Th1/Th2 平衡具有更明显的调节作用。 结论 复方甘草酸苷联合高频电刀治疗女性尖锐湿疣可提高疗效，通过调节患者Th1/Th2 细胞因子的平衡状态，改善患者的细胞免疫功能。

[关键词] 复方甘草酸苷；高频电刀；尖锐湿疣；Th1/Th2 细胞因子

[中图分类号] R711.7[文献标识码] B[文章编号] 1673—9701（2012）25—0070—03

The influence of compound glycyrrhizin plus high frequency radio—surgery procedures on Th1/Th2 related cytokines and curative effect of female condyloma acuminatum patients

XU Xuejuan1MA Xueliang2GUO Hongfei2

1.Department of Obstetrics and Gynecology， Tengzhou Central People′s Hospital Affiliated to Jining School of Medicine， Tengzhou 277500， China； 2.Department of Dermatology，Tengzhou Central People′s Hospital Affiliated to Jining School of Medicine， Tengzhou 277500， China

[Abstract] Objective To observe the influence of compound glycyrrhizin on Th1/Th2 related cytokines of female patients with condyloma acuminatum （CA） after high frequency radio—surgery procedures treatment. Methods Totally 70 cases of female CA were randomly divided into two groups. The first group was treated with compound glycyrrhizin after removed by high frequency radio—surgery procedures. The second group was only removed by high frequency radio—surgery procedures. In the groups above，the levels of IL—2， IL—4， IL—10 and IFN—γ in serum of female CA and control group were determined by ELISA before therapy and one month after therapy. Another control group was made up of twenty—five healthy cases accepting the same assays as the above. Results （1）The efficacy in the first treatment group was better than the second group（χ2=5.29， P < 0.05）；（2）Compared with the control group，the levels of IL—2 （t = 4.012， P < 0.05） and IFN—γ （t = 3.880， P < 0.05） in patients with female CA were lower before treatment； While the levels of IL—4（t = 2.694，P < 0.05） and IL—10 （t = 3.069， P < 0.05） were higher before treatment； （3）Compared with the second treatment group，the first treatment group has more significant regulatory role for Th1/Th2 related cytokines. Conclusion The compound glycyrrhizin plus high frequency radio—surgery procedures can improve efficacy and the immune function state by regulate the balance of Th1/Th2 related cytokines in the female CA.

[Key words] Compound glycyrrhizin； High frequency radio—surgery procedures； Condyloma acuminatum； Th1/Th2 related cytokines

尖锐湿疣（condyloma acuminatum，CA）是性传播疾病中发病率较高的疾病之一，是由人乳头状瘤病毒（human papillomavirus，HPV）引起的性传播疾病。目前普遍认为细胞免疫功能的变化与CA 的发生、转归密切相关。临床上通常用激光、冷冻、电灼或化学腐蚀等方法去除疣体，但治疗后易复发。为探讨治疗CA的有效方法和降低其复发率，我科应用复方甘草酸苷联合高频电刀治疗女性尖锐湿疣，同时检测患者外周血Th1/Th2 细胞因子的表达水平，现报道如下。

1 资料与方法

1.1 一般资料

选择2011年1～12月在我院妇科和皮肤性病科门诊诊治的尖锐湿疣患者70例，年龄21～53岁，平均43.8岁，诊断标准按照2003年卫生部制定的尖锐湿疣诊断标准[1]。排除近期患有其他急慢性感染性疾病患者、自身免疫性疾病患者、应激状态如妊娠、哺乳及长期服用避孕药等。将患者随机分为治疗1组与治疗2组各35例，两组患者在症状、年龄、病情程度等方面无显著性差异，具有可比性（P > 0.05）。对……