曹东旭，董海叶，李 妍，吕晓玲

(1. 天津科技大学食品工程与生物技术学院，天津 300457；2. 天津市食品加工工程中心，天津 300457)

紫甘薯花色苷对人肝癌细胞HepG2的作用

曹东旭1,2，董海叶1，李 妍1，吕晓玲1

(1. 天津科技大学食品工程与生物技术学院，天津 300457；2. 天津市食品加工工程中心，天津 300457)

以人肝癌细胞HepG2为模型研究紫甘薯花色苷的抗肿瘤活性．通过MTT实验检测紫甘薯花色苷对HepG2细胞的生长抑制作用，HE染色观察细胞形态，流式细胞仪测定细胞周期变化．MTT结果表明，不同浓度的紫甘薯花色苷处理HepG2细胞24、48、72,h后，低浓度的紫甘薯花色苷促进肿瘤细胞的生长，高浓度的紫甘薯花色苷抑制肿瘤细胞的生长，800,µg/m L花色苷处理72,h抑制率高达80%．HE染色结果表明，正常HepG2细胞的细胞核和细胞质清晰可见，而加入花色苷后，出现细胞核固缩、染色质浓集于核膜表面、形成新月形致密小斑块、细胞膜形态变得不规则等凋亡的特征．流式细胞仪分析结果表明，花色苷能够促进肿瘤细胞的凋亡，且细胞的凋亡率随花色苷浓度的增加而增大．

紫甘薯花色苷；HepG2细胞；抗肿瘤

Abstract：The anti-tumor activity of purple sweet potato anthocyanin to human liver cancer HepG2 cells was studied. Cell viability，morphology characteristic and cell cycle distribution were analyzed by MTT experiment，HE dyeing and flow cytometry，respectively. The MTT results indicated that after being treated w ith purple sweet potato anthocyanin for 24，48，72,h，the grow th of HepG2 cell was slightly promoted by low concentration of purple sweet potato anthocyanin and obviously inhibited by high concentration of purple sweet potato anthocyanin. The cell grow th inhibitory rate could reach up to 80% when HepG2 cells were treated w ith 800,µg/m L of anthocyanin for 72,h. The HE dyeing results indicated that the normal HepG2 cell nucleus and cytoplasm are clearly discernible，while after being treated w ith anthocyanin，HepG2 cells showed typical morphological changes associated w ith the characters of apoptosis，such as karyopyknosis，the chromatin enrich in the karyotheca surface，form ing the meniscus compact small mottling，the cell membrane shape becomes anomalous. Flow cytometry analysis results indicated that the anthocyanin could induce cell apoptosis，and the apoptotic percentage increased along w ith the anthocyanin concentration.

Keywords：purple sweet potato anthocyanin；HepG2 cells；anti-tumor

花色苷是一类广泛地存在于植物中的水溶性天然色素，是一大类多酚化合物的总称，也是植物的主要呈色物质[1]．何会等[2]研究了荔枝皮色素提取液中花色苷的抗氧化能力，分别测定花色苷的总抗氧化能力(TAC)，清除羟自由基、DPPH自由基、超氧阴离子自由基的能力，结果表明花色苷具有极佳的抗氧化能力．吴信子等[3]测定了花色苷的氧自由基吸收……