于志远, 吴仁安, 邹汉法＊

(1.中国科学院分离分析化学重点试验室,中国科学院大连化学物理研究所,国家色谱研究分析中心,辽宁大连116023;2.中国科学院研究生院,北京100049)

固定化牛血清白蛋白吸附材料的制备及其对胆红素的吸附性能

于志远1,2, 吴仁安1, 邹汉法1＊

(1.中国科学院分离分析化学重点试验室,中国科学院大连化学物理研究所,国家色谱研究分析中心,辽宁大连116023;2.中国科学院研究生院,北京100049)

降低高胆红素血症和重症肝炎患者血液中异常升高的胆红素浓度是血浆交换和血液灌流等疗法的目标之一。本文通过共价键合的方法将牛血清白蛋白(BSA)固定在甲基丙烯酸缩水甘油酯共聚三羟甲基丙烷三甲基丙烯酸酯[poly(GMA-co-TM PTMA)]大孔树脂微球上,制备得到对胆红素具良好吸附性能的固定化BSA吸附材料(B IA),吸附容量达48.7m g/g。由于血清白蛋白对胆红素的强烈相互作用,胆红素溶液中游离BSA的存在会显著降低B IA对胆红素的吸附量。B IA对胆红素的吸附量随吸附温度升高而增加。B IA在-80℃下储存31d后性能仍然稳定,对胆红素的吸附量几乎不变。上述结果表明所制备的B IA为以特异性吸附胆红素为目的的血液净化材料提供了新的选择。

吸附材料;牛血清白蛋白;微球;胆红素

Abstract:Serumbilirubin concentration is greatly elevated in certain diseases such as hyper biliru binemia and severe hepatitis.Lowering the level of bilirubin is one of the major targets of m any therapies such as plasma exchange and hemoper fusion.In this study,a bilirubin specific adsorbent was p repared by covalently immobilizing bovine serum album in(BSA)onto macroporouspoly(glycidyl methacrylate-co-trimethylol propanetrimethacrylate)microspheres.The resulting BSA immobilized adsorbent(B IA)demonstrated good perform ance in adsorp tion of bilirubin with an adsorption capacity of48.7m g/g.Presence of BSA in bilirubin solution significantly lowered the adsorption capacity of the adsorbent due to the tight binding of bilirubin onto BSA.Adsorption performance of the adsorbent for bilirubin was improved with the elevation of temperature.The adsorbent demonstrated good stability even after31d of storage at-80℃in that there was almost no change in adsorption capacity for bilirubin.These results indicated that the prepared BSA immobilized adsorbent could be an alternative choice for specific adsorption of bilirubin.

Key words:adsorbent;bovine serum album in;microspheres;bilirubin

胆红素(bilirubin)是血红素的代谢产物,在血浆中它与血清白蛋白结合并被运送到肝脏进行代谢[1]。血浆内胆红素浓度是肝功能检查的一项重要生理指标,它反映了肝脏吸收、代谢胆红素的能力。在新生儿高胆红素……