徐锦萍, 何 珩, 徐梦依, 曲燕华

(1.华中科技大学同济医学院药学院,湖北武汉430030;2.武汉工程大学邮电与信息工程学院,湖北武汉430073)

反相高效液相色谱法测定饲料添加剂阿散酸中的阿散酸及掺假物质对氨基苯磺酸

徐锦萍1＊, 何 珩2, 徐梦依1, 曲燕华1

(1.华中科技大学同济医学院药学院,湖北武汉430030;2.武汉工程大学邮电与信息工程学院,湖北武汉430073)

建立了用于饲料添加剂阿散酸质量控制的反相高效液相色谱法。采用的色谱条件:Waters Bondapak C18柱(150mm×4mm,5μm)分离,以甲醇-水(用稀磷酸调节pH至2.9)(1∶4,v/v)作流动相,流速1.0mL/m in,紫外检测波长为244nm。在优化的色谱条件下,阿散酸和掺假物质对氨基苯磺酸在3m in内实现了基线分离。阿散酸和对氨基苯磺酸的线性范围均为5～200m g/L,检出限(S/N=3)分别为0.20m g/L和0.15m g/L。该方法简便快速,适合饲料添加剂阿散酸的分析以及监测对氨基苯磺酸掺假。

反相高效液相色谱法;阿散酸;对氨基苯磺酸;掺假物质;饲料添加剂

Abstract:A reversed-phase high performance liquid Chromatographic(RP-HPLC)method was established for the determination of arsanilic acid and sulfanilic acid as adulterant in the feed additives.The separation was carried out on a Waters Bondapak C18column,and methanol water(pH2.9adjusted by0.01mol/L phosphoric acid)(1∶4,v/v)was used as the mobile phase with a flow rate of1.0mL/m in.A diode array detector was used at244nm as the detection wavelength.Arsanilic acid and sulfanilic acid were separated within3m in.The linear ranges all were5-200m g/L and the detection limits(S/N=3)were0.20and0.15m g/L for arsanilic acid and sulfanilic acid,respectively.This method is simple and rap id,and suitable for the simultaneous determination of arsanilic acid and sulfanilic acid in feed additives.

Key words:reversed-phase high perform anceliquid Chromatography(RP-HPLC);arsanilic acid;sulfanilic acid;adulterant;feed additive

阿散酸,化学名称为对氨基苯砷酸,是猪、鸡饲料的添加剂,具有促进蛋白质的合成,改变肠道细胞的代谢,增进皮肤卵黄素沉淀,并能抑制球虫卵、支原体,抗大肠杆菌,起着生长促进剂的作用,能提高产蛋率和饲料效率[1]。目前市场上以阿散酸和阿散酸-碳酸钙预混剂产品为主。近来,市场上发现有不法厂商在阿散酸添加剂中掺入更加价廉易得的非功能性物质(如对氨基苯磺酸),以提高阿散酸的表观含量,获取不当利益。

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