基于代谢组揭示雪兔和妙香7号草莓果实香气物质的差异
2025-07-08乔慧兰马铭泽武爱园豆志琦祝秀梅马宗桓
Abstract:To clarify the diferences in aroma compounds between white strawberry Snow Rabbit and red strawberry Miaoxiang No.7,thebasic fruit qualityandaroma compoundsofthe two varietiesat the maturitystage were determined in this study.Basicqualitiesincluding singlefruit weight,anthocyanincontent,solublesolidscontent,andtotal phenolicontent were measured. Gas chromatography-mass spectrometry(GC-MS)was employed to detect volatilecompounds in the fruitsof thetwostrawberryvarieties.TheresultsshowedthatthefruitshapeindexofMiaoxiangNo.7was1.41,thefruitwas
longconicalinshape,whilethefruitshapeindexofSnow Rabbit was1.15,and the fruitwasshort conical in shape. The total phenolic content and anthocyanin content of Miaoxiang No.7 were 4.10mg/g and 1.21mg/g ,respectively,both were significantly higher than those of Snow Rabbit.A total of 471 metabolitesin15 categorieswere detected in the two strawberry cultivars,of which117 were upregulated,24weredown-regulated,and 96weredifferentiallyexpressed metabolites,accounting for 20.38% ofthe
totalmetabolites.Theeightdiferentialmetaboliteswithhighquantitieswerealcohols,aldehydes,acids,terpenoids,hydro carbons,ketones,heterocycliccompounds,and esters.These compounds were mainlyenriched inthebiosynthetic pathway of terpenoids.Themainvolatilecompoundsin SnowRabbitstrawberry fruitwereesters,whilethosein MiaoxiangNo.7fruit were terpenoids.There were 2O characteristicaroma compounds in Snow Rabbit strawberry,with a herbaceous aroma type, and there were 22 characteristic aroma compounds in Miaoxiang No.7 strawberry,with a fruity aroma type.
Keywords:strawberry;fruit;differential metabolites;aroma substances;quality
草莓(Fragaria × ananassa Duch.)属于蔷薇科(Rosaceae)草莓属(Fragaria)[1],是多年生草本浆果类果树,其果实通常呈现出鲜艳的红色,然而,除了常见的红色果实之外,也有一部分草莓的果实为白色。草莓具有极高的经济价值和营养价值,在市场上备受欢迎,有“水果皇后”之称。近年来随着农业技术的不断进步以及市场需求的持续增长,中国草莓产业快速发展,种植面积不断扩大,种植技术日益成熟,草莓的产量也在稳步提升。如今,中国已成为世界第一草莓生产和消费大国[2]。改善草莓果实品质是当前草莓产业发展的任务[3],在草莓的众多品质中,香气堪称一项至关重要的品质,它不但是评价草莓品质的关键指标,同时也是草莓在育种、鲜食以及加工等方面的重要考量因素[4]。香气由植物细胞中的挥发性有机物(VOCs)产生,占鲜重的0.001%~0.010% ,包括酯类、萜类、醚类、烷烃、苯等物质[5]。这些挥发性化合物共同作用,混合形成了草莓果实独特的香气。草莓果实的香气呈现出多种不同的类型,主要的香气类型有水果香味、青草香味、柑橘香味、焦糖香味、黄油味以及花香味。草莓的香气类型与其香气活性值(实际浓度/香气阈值)有关,某种挥发性化合物的香气活性值大于1代表其特征性香气物质能够对其香气类型作出贡献。草莓的焦糖香由呋喃类化合物的味道构成,呋喃类化合物是新鲜草莓特有的挥发性化合物。尽管草莓中的呋喃酮含量极少,然而其香气阈值较低,对草莓的香气起着重要的作用[6-7]。……
