刘静 李春霞 何巧玉 王誉程 石玉梦 刘二伟 陈晓鹏

摘要 目的：基于中藥质量标志物（Q-marker）的概念，建立高效液相色谱法（HPLC）指纹图谱，并测定2种成分的含量，同时应用网络药理学方法探讨2种成分的潜在作用靶点进行反向筛选，从而确定黄芩药材的质量标志物。方法：采用HPLC建立10批黄芩药材的指纹图谱，并进行含量测定。结合网络药理学分析有效成分的对应靶点和途径并构建“活性成分-重要靶点-通路”，探究黄芩发挥药理作用特点，预测出潜在的Q-marker。结果：建立的黄芩药材指纹图谱共标定12个共有峰，指认2个成分并确定其含量。10批样品指纹图谱与对照图谱的相似度均大于0.970。“化合物-预测靶点”网络筛选出8个关键靶点，相关9条通路，涉及到癌症通路、炎症通路、免疫通路等。结论：本研究所建立的HPLC准确、可行，筛选出2种成分可作为潜在Q-marker，以网络药理学验证黄芩主成分相关的靶点作用机制，进一步确认黄芩中黄芩苷、汉黄芩苷作为Q-marker的可行性，为黄芩质量控制提供参考。

关键词 黄芩;质量标志物;高效液相色谱法;指纹图谱;含量测定;黄芩苷;汉黄芩苷;网络药理学

Prediction of Q-markers for Radix Scutellariae Based on Fingerprint and Network Pharmacology

LIU Jing，LI Chunxia，HE Qiaoyu，WANG Yucheng，SHI Yumeng，LIU Erwei，CHEN Xiaopeng

（State Key Laboratory of Component-based Chinese Medicine，Tianjin University of Traditional Chinese Medicine，Tianjin 301617，China）

Abstract Objective：Based on the concept of Q-marker，high performance liquid chromatography（HPLC） fingerprints were established for Radix Scutellariae and the content of two components was determined.Via network pharmacology，the potential targets of the two components were predicted for determining the Q-markers of the medicinal.Methods：The HPLC fingerprints of 10 batches of Radix Scutellariae were established，and the content of baicalin and wogonoside was determined.Based on network pharmacology，the targets of the active components and related pathways were analyzed and the “component-target-pathway” network was plotted.Thereby，pharmacological characteristics of Radix Scutellariae were clarified and the potential Q-markers were forecasted.Results：The fingerprints of Radix Scutellariae shared 12 peaks.Two of them were identified，namely，baicalin and wogonoside，and the content was determined.The fingerprints of the 10 batches of samples showed>0.970 similarity to the reference fingerprint.A total of 8 key targets which were involved in 9 pathways such as inflammation pathway，cancer pathway，and immune pathway，were screened out.Conclusion：The HPLC method is accurate and reliable，and two components can be used as potential Q-markers of Radix Scutellariae.The mechanism related to the principal components of Radix Scutellariae was further tested by network pharmacology，which confirmed that baicalin and wogonoside can be used as Q-markers of the medicinal.The result is expected to serve as a reference for quality control of Radix Scutellariae.

Keywords Radix Scutellariae; Quality marker; High performance liquid chromatography; Fingerprints; Determination of content; Baicalin; Wogonoside; Network pharmacology6CF65330-F464-4784-8903-A8464DAF226F

中图分类号：R285;R284文献标识码：Adoi：10.3969/j.issn.1673-7202.2022.08.001

中药饮片作为中药制药的原材料，其质量的好坏直接关系到中医临床用药的安全性与有效性，并直接影响到患者的健康甚至生命安全。确保中药饮片质量的安全性和有效性极为重要。质量标志物（Q-marker）是中药质量控制的新概念[1]，基于成分的特有性、可测性、有效性、质量传递与溯源及配伍环境5个方面，建立中药饮片的Q-marker更加有利于中药饮片的全面质量控制和追溯体系，从而提高反映饮片特异性的质量标准。