林庆宾 陈鹏 刘宇 李楠

〔摘要〕 目的 觀察补肾活血汤对脊髓损伤（spinal cord injury， SCI）大鼠后肢功能、脊髓组织病理损伤，及对Tau、BDNF、NT-3、NGF蛋白表达的影响，探讨其作用机制。方法 24只SD大鼠随机分为假手术组、模型组、补肾活血汤组，每组8只。模型组、补肾活血汤组大鼠采用改良Allen’s法建立大鼠SCI模型，假手术组手术操作同模型组，但不打击脊髓。补肾活血汤组大鼠用补肾活血汤以10.8 g/（kg·d）剂量灌胃，假手术组和模型组大鼠用等量生理盐水灌胃。在模型建立后第1、3、7、14、28天用巴索、比蒂和布雷斯纳汉运动评级（Basso， Beattie & Bresnahan locomotor rating， BBB）法评估大鼠后肢功能;第28天观察结束后进行脊髓组织取材，尼氏染色观察脊髓组织病理结构;用Western blot和免疫组化法检测脊髓组织Tau、BDNF、NT-3、NGF蛋白的表达。结果 （1）与模型组相比，补肾活血汤组大鼠BBB评分在第14、28天明显增高（P<0.05）;（2）补肾活血汤组脊髓组织神经元数目（P<0.05）和形态优于模型组;（3）Western blot和免疫组化结果显示，与模型组相比，补肾活血汤脊髓组织Tau蛋白表达显著降低（P<0.05），BDNF、NT-3、NGF的表达显著增高（P<0.05或P<0.01）。结论 补肾活血汤可改善SCI大鼠肢体功能，减轻脊髓组织病理损伤，其作用机制可能与抑制Tau蛋白表达，增加BDNF、NT-3、NGF蛋白的表达有关。

〔关键词〕 补肾活血汤;脊髓损伤;Tau;BDNF;NT-3;NGF

〔中图分类号〕R285.5       〔文献标志码〕A        〔文章编号〕doi：10.3969/j.issn.1674-070X.2021.12.007

Effect of Bushenhuoxue Decoction on Tau Protein and Neurotrophic Factor Expression in

Rats with Spinal Cord Injury

LIN Qingbin1，2， CHEN Peng1，2， LIU Yu1，2， LI Nan1，2

（1. Fujian University of Traditional Chinese Medicine， Fuzhou， Fujian 350122， China; 2. Key Laboratory of Orthopedics & Traumatology of Traditional Chinese Medicine and Rehabilitation， Ministry of Education， Fujian University of Traditional

Chinese Medicine， Fuzhou， Fujian 350122， China）

〔Abstract〕  Objective To observe the effects of Bushenhuoxue Decoction on limb function， pathological structure of spinal cord， expression of Tau， BDNF， NT-3 and NGF in spinal cord injury （SCI） rats， and to explore its effect mechanism. Methods 24 SD rats were randomly divided into sham operation group， model group and Bushenhuoxue Decoction group， with 8 rats in each group. SCI model was established by modified Allen’s method in the model group and Bushenhuoxue Decoction group. Operation in the sham operation group was the same as that of the model group without spinal cord attack. Rats in the Bushenhuoxue Decoction group were given the Bushenhuoxue Decoction at 10.8 g/（kg·d） dose while sham operation group and the model group were given the same amount of normal saline. Basso， Beattie & Bresnahan locomotor rating （BBB） method was used to evaluate the hind limb function of rats at 1， 3， 7， 14， and 28 days after model establishment. Spinal cord tissue samples were taken after the observation on day 28. The pathological structure of the spinal cord was observed by Nissl staining， and the expression levels of Tau， BDNF， NT-3 and NGF in the spinal cord were detected by Western blot and immunohistochemistry. Results （1） Compared with model group， BBB scores of the rats in the Bushenhuoxue Decoction group were significantly increased at 14th and 28th day （P<0.05）. （2） The number （P<0.05） and morphology of neurons of spinal cord in Bushenhuoxue Decoction group were superior than those in model group. （3） Western blot and immunohistochemical results showed that， compared with the model group， the expression of Tau protein in the spinal cord of Bushenhuoxue Decoction group was significantly decreased， and the expression levels of BDNF， NT-3 and NGF were significantly increased （P<0.05 or P<0.01）. Conclusion Bushenhuoxue Decoction can improve limb function and relieve pathological injury of spinal cord in SCI rats， and its mechanism may be related to inhibiting Tau protein expression and increasing the expression of BDNF， NT-3 and NGF proteins.

〔Keywords〕 Bushenhuoxue Decoction; spinal cord injury; Tau; BDNF; NT-3; NGF

脊髓损伤（spinal cord injury， SCI）属于严重的中枢神经系统损伤，是脊柱骨折最严重的并发症之一，可导致损伤平面以下运动、感觉和自主神经功能障碍[1]。SCI具有高致死率和致残性的特点，全世界因创伤导致的SCI发病率约为3.6～195.4人次/100万[2]。中国SCI发病率约为37人次/100万，而且好发人群集中在青壮年，不仅给患者本人造成严重伤害，也给家庭和社会带来沉重的负担[3]。……