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基于黄水酯化产酯酵母的筛选

2021-11-26陈雪玲兰小艳廖诚陈惠

安徽农业科学 2021年21期

陈雪玲 兰小艳 廖诚 陈惠

摘要 为得到可用于黄水酯化的产酯酵母,以四大酯为评价指标,将分离自浓香型白酒产区的17株酵母先通过改良酸醇酯化体系进行产酯能力初筛,再将初筛得到的7株酵母应用于黄水酯化进行复筛。结果表明:在改良酸醇酯化体系中有16株酵母能促进己酸乙酯合成,有7株与对照差异显著;7株酵母均能提高黄水己酸乙酯,其中H1Y-24与对照差异极显著,并且能降低乳酸乙酯,使酯化液四大酯量比关系更加协调。

关键词 黄水;酵母;产酯能力;己酸乙酯

中图分类号 TS 261.9  文献标识码 A  文章编号 0517-6611(2021)21-0174-03

doi:10.3969/j.issn.0517-6611.2021.21.044

开放科学(资源服务)标识码(OSID):

Screening of Ester-producing Yeast in Yellow Water Esterification

CHEN Xue-ling  LAN Xiao-yan  LIAO Cheng2 et al

(1.Yibin Vocational and Technical College, Yibin, Sichuan 644003; 2.Nanguang Primary School in Xuzhou District of Yibin City, Yibin, Sichuan 644000)

Abstract In order to obtain ester producing yeast for yellow water esterification,four esters were used as evaluation indexes, seventeen strains of yeast isolated from Luzhou flavor liquor producing areas were screened for ester production capacity through improved acid alcohol esterification system,then the seven yeast strains were used in yellow water esterification for rescreening. The results showed that in the improved acid alcohol esterification system, 16 strains of yeast could promote the synthesis of ethyl caproate, and 7 strains were significantly different from the control. The results showed that all the seven yeast strains could increase ethyl hexanoate in yellow water, and the difference between H1Y-24 and the control was significant, and could reduce ethyl lactate and higher alcohols, making the relationship between the four ester ratio of esterification liquid more harmonious.

Key words Yellow serofluid;Yeast;Ester production capacity;Ethyl hexanoate

基金項目 宜宾职业技术学院院级一般项目(ZRKY21YB-1 ZRKY21YB-10);宜宾职业技术学院院级重点项目(ybzysc20-01)。

作者简介 陈雪玲(1985—),女,四川安岳人,讲师,硕士,从事微生物发酵研究。

收稿日期 2021-01-28;修回日期 2021-05-28

黄水是浓香型白酒酿造过程特有的副产物,其产量大,物质丰富,直接排放将造成资源浪费及环境污染[1]。向黄水中添加产香微生物将其发酵为黄水酯化液用于白酒生产,是目前黄水资源再利用的主要手段[2]。现有研究表明,白酒企业目前所用黄水酯化体系较为复杂,一般有大曲、酯化酶、窖泥、酒尾等物质,导致酯化结果重现性差,且酯化时间较长,设备利用率低[3]。

该研究将分离筛选自宜宾白酒产区的产香酵母制成麸曲粗酶制剂,以己酸乙酯生成量为主,其他风味物质为辅作检测指标,先通过改良的混酸醇酯化体系进行酯化力初筛,再将筛选所得的酵母应用于黄水酯化力筛选,以期得到可应用于黄水生香的潜在酵母,同时为浓香型白酒企业制备黄水酯化液提供参考。……

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