徐礼臻　徐旻　朱再生

[关键词] 前列腺癌;PTEN 蛋白;Eg5 蛋白;免疫组织化学

[中图分类号] R737.2          [文献标识码] A          [文章编号] 1673-9701（2021）17-0004-04

Expression and correlation of PTEN and Eg5 protein in prostate cancer

XU Lizhen   XU Min   ZHU Zaisheng

Department of Urology， Jinhua Hospital Affiliated to Zhejiang University Medical College， Jinhua   321000， China

[Abstract] Objective To detect PTEN and Eg5 protein in prostate cancer（PC） by immunohistochemistry， and to explore the correlation between the expression of PTEN and Eg5 protein and clinicopathology of prostate cancer. Methods Immunohistochemical SP method was used to detect the expressions of PTEN and Eg5 protein in 81 PC cases and 45 cases with prostatic hyperplasia in Jinhua Hospital Affiliated to Zhejiang University School of Medicine from December 2014 to March 2016. The clinicopathological data of PC patients were analyzed with PTEN and Eg5 expression. Spearmen was used to analyze the correlation between PTEN and Eg5 protein expression. Results The positive rate of PTEN protein expression was 25.93% （21/81） in PC and 88.89% （40/45） in benign prostatic hyperplasia （BPH）， with statistically significant difference （P<0.05）. The positive rate of Eg5 protein was 70.37%（57/81） in PC and 4.44% （2/45） in BPH， with statistically significant difference（P<0.05）. The loss of PTEN protein expression and the increased expression of Eg5 protein were closely related to Gleason score， TNM stage， preoperative PSA， seminal vesicle invasion， pelvic lymph node metastasis and five-year biochemical recurrence of PC （P<0.05）. Spearmen correlation analysis showed that the expression of PTEN was negatively correlated with the expression of Eg5 protein（r=-0.763， P<0.05）. Conclusion The expression of PTEN protein decreases and Eg5 protein increases in PC， and their abnormal expressions are related to the progression of PC.

[Key words] Prostate cancer; PTEN protein; Eg5 protein; Immunohistochemistry

PTEN基因（Phosphatase and tensin homologue deleted on chromosome ten，PTEN）為具有脂质磷酸酶和蛋白磷酸酶活性的抑癌基因，位于10号染色体上[1-2]，研究显示PTEN去磷酸化在肿瘤细胞增殖、分化、浸润、转移及肿瘤血管形成中有重要的作用[3]。Eg5（Kinesin spindle protein 5，Eg5）基因位于10q24.1，其翻译的蛋白产物由1057个氨基酸组成，在细胞有丝分裂过程中纺锤体定位、形成、染色体分离和细胞质分裂中起重要作用[4-5]，异常表达干扰正常纺锤体的形成和功能，导致遗传不稳定和肿瘤发生[6-7]。虽然在前列腺癌中PTEN蛋白异常表达与其临床病理特征关系有部分研究，但PTEN和Eg5蛋白在前列腺癌中的异常表达相关性未见报道，本研究采用免疫组化SP（Streptavidin method，SP）法对PTEN和Eg5蛋白在前列腺癌中的表达及临床病理相关性及生化复发之间的关系进行研究，现报道如下。

1 资料与方法

1.1 一般资料

选取2014年12月至2016年3月浙江大学医学院附属金华医院81例前列腺癌患者，年龄51～83岁，中位年龄66.5岁，同时选取同期45例前列腺增生患者，所有患者的病理均经两名有经验的病理医师再次确认诊断。前列腺癌病理分级按Gleason分级系统[8]，Gleason评分<7分45例，Gleason评分≥7分36例。临床分期按2002年AJCC的TNM分期标准[9]，Ⅰ～Ⅱ期42例，Ⅲ～Ⅳ期39例。术前49例PSA（Prostate specific antigen，PSA）4～10 ng/mL，32例PSA>10 ng/mL，有精囊浸潤21例，无精囊浸润60例，18例有盆腔淋巴结转移，63例无盆腔淋巴结转移，5年有生化复发37例，5年无生化复发44例，所有前列腺癌患者术前均未接受放疗、化疗和内分泌治疗。本研究经本院医学伦理委员会同意，患者签署知情同意书。

1.2 方法

研究组织标本经4%甲醛溶液固定，常规石蜡包埋，连续……