宋春艳 马思佳 孟宇鹏 孙耕天 钱明

摘 要：牙周牙髓疾病严重影响人们正常工作和生活。常规治疗方法是牙周洁治、刮治、根面平整和根管治疗。由于其在恢复正常牙体组织方面存在一定的局限性，因此基于组织工程的牙再生技术应运而生。牙再生是通过干细胞的分离、培养、诱导分化，并最终形成部分或完整牙齿结构的过程，在实际临床应用中意义非凡。近年来，3D生物打印技术受到国内外研究学者的青睐，其可跳過细胞接种程序，直接将细胞和生物材料精确放置在三维结构内的预定位置，在组织工程领域应用广泛。目前，越来越多的学者将3D生物打印用于牙再生领域的研究中。基于此，文章归纳总结了3D生物打印在牙再生领域中的应用，希望对今后的相关研究提供一些理论指导。

关键词：生物打印;牙再生;组织工程

中图分类号：R78  文献标识码：A     文章编号：1001-5922（2021）10-0081-05

The Research Progress of 3D Bioprinting in Tooth Regeneration

Song Chunyan， Ma Sijia， Meng Yupeng， Sun Gengtian， Qian Ming

（Department of Prosthodontics， Stomatological Hospital of Jilin University， Changchun 130021， China ）

Abstract：Periodontal pulp disease seriously affects peoples normal work and life. The conventional treatment methods are periodontal cleansing， curettage， root planing and root canal treatment. Due to its limitations in restoring normal tooth tissues， tooth regeneration techniques based on tissue engineering have emerged. Tooth regeneration is the process of separating， culturing， and inducing differentiation of stem cells， and finally forming a partial or complete tooth structure， which is of great significance in practical clinical applications. In recent years， 3D bioprinting technology has been favored by research scholars at home and abroad. It can skip the cell seeding procedure and directly place cells and biological materials at predetermined positions within the three-dimensional structure. It is widely used in the field of tissue engineering. At present， more and more scholars are using 3D bioprinting for research in the field of tooth regeneration. Based on this， this paper summarizes the application of 3D bioprinting in the field of tooth regeneration， hoping to provide some theoretical guidance for related research in the future.

Key words：bioprinting;tooth regeneration;tissue engineering

牙齿在人的一生中承担着重要功能，不仅可以维持美观，还参与咀嚼、消化、营养吸收等活动。而感染、外伤、先天疾病常常导致牙周牙髓疾病，前者可引起牙龈出血、牙槽骨吸收，甚至牙齿松动、脱落，而后者可造成牙齿变色、疼痛。有研究表明，牙周疾病还与多种全身疾病相关，包括糖尿病、心血管疾病、慢性呼吸系统疾病、癌症等[1-2]。传统治疗方法存在一定的不足：常规牙周治疗只能阻止疾病的发生发展，不能再生牙周组织;而根管治疗会导致牙齿失去营养支持，脆性增加，进而造成牙折的发生率大大上升。……