张维明　李立纪　陶剑　陈得玲　唐世朗

摘要：目的 探讨傣药豆腐渣果树皮和果实95%乙醇提取物对酒精引起的氧化应激损伤的抗氧化应激及抗炎活性。方法 采用采用56度北京红星二锅头白酒，以0.05mL/10 g.d给KM种小鼠连续灌胃4周，于模型复制的第2周开始，动物在造模的同时分别给予豆腐渣果树皮和果实95%乙醇提取物，连续给予3周，末次给药后，处死动物，检测动物血清中MDA、SOD、TNF-α的含量及脑匀浆中MDA、SOD的含量。结果 与正常组比较，模型组小鼠给予白酒后第4周即出现血清和脑组织中SOD水平明显下降（P<0.01），MDA水平明显升高（P<0.01），血清中炎症因子TNF-α水平明显升高（P<0.01）。与模型组相比，给予豆腐渣果树皮和果实的醇提取物3周后，均可降低血清中MDA水平和TNF-α水平（P<0.05），并可升高血清和脑中SOD水平（P<0.05）。结论 豆腐渣果树皮和果实的95%乙醇提取物可减轻酒精引起的氧化应激损伤及炎症因子释放。

关键词：豆腐渣果;抗氧化;抗炎;活性研究

中图分类号：R285.5   文献标志码：A   文章编号：1007-2349（2021）10-0075-04

【Abstract】Objective： To study the anti-oxidative stress and anti-inflammatory activities of the 95% ethanol extracts from the bark and fruit of Helicia erratica Hook in Dai medicine on alcohol-induced oxidative stress damage. Methods： 56% degree of Beijing Hongxing Erguotou Liquor were used to give KM mice intragastrically with 0.05mL/10 god for 4 consecutive weeks. Starting from the second week of model replication， the animals were given the 95% ethanol extracts from the bark and fruit of Helicia erratica Hook for 3 weeks at the same time as the model was created. After the last administration， the animals were sacrificed to detect the contents of MDA， SOD， and TNF-α in the animal serum and the contents of MDA and SOD in the brain homogenate. Results： Compared with the normal group， the SOD level in serum and brain tissue of the model group decreased significantly （P<0.01）， the MDA level increased （P<0.01）， and the serum inflammatory factor TNF-α level in the 4th week after the administration of liquor was significantly increased （P<0.01）. Compared with the model group， after 3 weeks of alcohol extracts from the bark and fruit of Helicia erratica Hook， the levels of MDA and TNF-α in the serum could be reduced （P<0.05）， and the levels of SOD in the serum and brain could be increased （P<0.05）. Conclusion： The 95% ethanol extract from the bark and fruit of Helicia erratica Hook can reduce the oxidative stress damage and the release of inflammatory factors caused by alcohol.

【Key words】Helicia erratica Hook; Antioxidant; Anti-inflammation; Activity Study

酒精中毒性腦病（Alcoholic Brain Disease，ABD）是酒精中毒最常见、也是最严重的并发症之一，是由于长时期大量酗酒引起人体营养代谢紊乱，导致中枢神经损害的一类疾病。肝脏是酒精代谢的主要器官，因此既往对酒精造成肝脏损伤的研究较为深入，但近年来的研究表明，过度饮酒可造成脑内炎症甚至是不可逆性脑组织损伤[1]，因此ABD的诊治越来越引起人们的关注。

豆腐渣果（深绿山龙眼，Helicia nilagirica Bedd）为山龙眼科Proteaceae山龙眼属Helicia植物，分布于云南产于云南西南部和南部（西双版纳、普洱、保山等地），是傣医用于解除农药中毒、食用野生菌中毒的常用民族药。课题组前期研究发现，豆腐渣果的乙醇提取物具有一定的解酒精中毒作用，并能减轻急性脑损伤动物脑组织炎症反应，故推测豆腐渣果可能对ABD具有潜在活性，本研究拟探讨豆腐渣果不同部位的95%乙醇提取物对酒精诱导的氧化应激反应和炎症因子释放的影响，为研究其对抗ABD的活性提供依据。……