李光宁， 程文超， 胡荣娟， 强胜， 夏爱萍， 左娇， 宋小玲， 张瑞萍

摘要：為探究0.136%赤·吲乙·芸薹可湿性粉剂（WP）与除草剂氯氟吡啶酯混用对稗草防除效果及生理生化的影响，以无芒稗为研究对象，设置清水对照、0.136%赤·吲乙·芸薹可湿性粉剂45 g/hm2、3%氯氟吡啶酯乳油（EC）1 200 mL/hm2和0.136%赤·吲乙·芸薹WP 45 g/hm2+3%氯氟吡啶酯EC 1 200 mL/hm2 4个处理，观察药后无芒稗的药害症状，测定叶绿素荧光参数及糖代谢和氮代谢等生理指标。药后7 d，与氯氟吡啶酯单用相比，赤·吲乙·芸薹与氯氟吡啶酯混用，无芒稗药害综合指数显著提高26.6%，Fv/Fm、ETR、qP均显著下降;蔗糖合酶活性、蔗糖磷酸合酶活性、全氮含量、硝酸还原酶活性、谷氨酰胺合成酶活性和谷氨酸合酶活性也均显著下降;脱落酸显著提高。这表明赤·吲乙·芸薹与氯氟吡啶酯混用后，无芒稗光合作用及糖代谢和氮代谢受抑制程度加强，提高了氯氟吡啶酯对无芒稗的防治效果。

关键词：赤·吲乙·芸薹;氯氟吡啶酯;无芒稗;水稻;增效

中图分类号：S451文献标志码：A文章编号：1003-935X（2021）02-0047-11

Effect of GA·IAA·BR 0.136% WP Mixed with

Florpyrauxifen-Benzyl on Control Efficiency and Physiological and

Biochemical Characteristics of Echinochloa crusgali var. mitisLI Guangning1，CHENG Wenchao1，HU Rongjuan2，QIANG Sheng1，

XIA Aiping2，ZUO Jiao2，SONG Xiaoling1，ZHANG Ruiping2，3

（1.Weed Research Lab，College of Life Sciences，Nanjing Agricultural University，Nanjing 210095，China;

2.Beijing Plum Agrochemical Trading Co.，Ltd.，Beijing 100025，China;

3.Jiangsu AgraForUm Soil Remediation Co.，Ltd.，Nantong 226300，China）Abstract：The effect of GA·IAA·BR 0.136% WP mixed with florpyrauxifen-benzyl on control efficiency and physiological and biochemical characteristics of Echinochloa crusgali var. mitis was studied in paddy fields. There were four treatments including water as a control （CK），GA·IAA·BR 0.136% WP at 45 g/hm2，florpyrauxifen-benzyl 3% EC at 1 200 mL/hm2 and the mixture of both at the same doses. Herbicide damage symptoms were observed and chlorophyll fluorescence parameters and physiological indexes including glucose metabolism and nitrogen metabolism were determined. Compared with single application of florpyrauxifen-benzyl，the comprehensive index of herbicide damage to E. crusgalli var. mitis significantly increased by 27%，but the Fv/Fm，ETR and qP decreased significantly 7 days after the mixture of both products was. The activity of sucrose synthase，sucrose phosphate synthase，nitrate reductase，glutamine synthetase and glutamate synthase and content of total nitrogen decreased significantly，but the content of abscisic acid increased significantly. This indicated that the inhibition of photosynthesis，glucose metabolism and nitrogen metabolism of E. crusgalli var. mitis was strengthened when GA·IAA·BR 0.136% WP mixed with florpyrauxifen-benzyl were applied thus improving the efficacy of florpyrauxifen-benzyl.

Key words：0.136%GA·IAA·BR;florpyrauxifen-benzyl;Echinochloa crusgali var. mitis;Oryza sativa;synergism

植物生长调节剂具有调节植物体内物质的输导和生长发育、新陈代谢的功能，因而可提高防除杂草的效果[1-4]，同时减轻对作物的药害[5-6]。0136%赤·吲乙·芸薹可湿性粉剂（商品名为碧护）是由德国科学家研究开发的植物源复合平衡型植物生长调节剂，内含赤霉素0.135%、芸薹素内酯0.000 31%、吲哚乙酸0.000 52%、脱落酸、茉莉酮酸等多种天然植物内源激素，10余种黄酮类催化平衡成分和近20种氨基酸及抗逆诱导剂等，能够诱导作物提高抗逆性和产量、改善品质、缓解药害[7]，被认为是首批商业化的天然植调剂之一[8]。……