彭绿春 宋杰 李树发 蔡艳飞 瞿素萍 田歌 李世峰

摘 要：为解决木本切花植物帝萝花‘璀璨明珠繁殖效率低的问题，该文以帝萝花‘璀璨明珠的幼嫩枝芽为外植体，研究了不同基本培养基对其长势的影响、不同激素种类和浓度对其增殖和生根的效果，分析了其离体繁殖的生长特点，并建立了高效的帝萝花‘璀璨明珠组培快繁技术体系。结果表明：帝萝花‘璀璨明珠幼嫩枝芽的消毒方法为0.1%的升汞溶液浸泡12 min，污染率为21.5%;外植体在WPM+ZT 1 mg·L-1+NAA 0.1 mg·L-1培养基上，侧芽萌发率为73%;增殖的最佳培养基为MS + BA 0.4 mg·L-1 + NAA 0.05 mg·L-1，增殖系数为6.63，增殖方式为侧芽增殖和植株基部丛生芽增殖; 生根的适宜培养基为MS + IBA 0.75 mg·L-1+ NAA 1 mg·L-1，生根率为70%;生根瓶苗移栽于珍珠岩和细草炭（体积比为0.5∶1）的基质中，光照强度为10 000～12 000 lx，空气湿度为70%～80%下培养，60 d后成活率可达72%。该研究结果为帝萝花组培种苗的商业化生产提供了技术支撑，同时促进了该高档木本切花的推广和种植及产业化。

关键词：帝萝花‘璀璨明珠，木本切花，植株高效再生，组培快繁

中图分类号：Q943

文献标识码：A

文章编号：1000-3142（2021）09-1542-06

Abstract：In order to improve reproduction efficiency of woody cut-flower plant Telopea speciosissima ‘Braidwood Brilliant，we used young branches and shoots as explants to reveal the growth characteristics of T. speciosissima ‘Braidwood Brilliantin in vitro culture and to establish its efficiently regeneration system，by studying the influence of basic medium on plant growth，the effect of hormone type，concentration on proliferation and rooting. The results were as follows：Treating explants with the 0.1% HgCl2 for 12 min，the explants contaminate rate was 21.5%; The lateral buds germination rate of explants was 73% on medium WPM+ZT 1 mg·L-1+NAA 0.1 mg·L-1; The optimal multiplication medium was MS + BA 0.4 mg·L-1+ NAA 0.05 mg·L-1with 6.63 proliferation coefficient，and the proliferating shoots originating from axillary bud and basic adventitious bud; MS + IBA 0.75 mg·L-1+ NAA 1 mg·L-1 was the suitable rooting medium，with 70% rooting rate; The rooting seedlings were transplanted in the substrates[perlite∶moss（v∶v）=0.5∶1] in the greenhouse with light intensity 10 000-12 000 lx，air humidity 70%-80%，and the survival rate of the seedlings was 72% 60 d later. The results provide technological support for commercial production of Telopea speciosissima tissue culture seedlings，and promoted the planting and industrialization of this famous woody cut-flower.

Key words：Telopea speciosissima ‘Braidwood Brilliant，woody cut-flower，plant efficient regeneration，tissue culture speed propagation

帝萝花（Telopea speciosissima）是山龍眼科（Proteaceae）帝萝花属（Telopea）多年生名贵木本观赏植物，原产于澳大利亚和非洲热带地区，其花形独特、花色艳丽、花期较长，既可用作切花，又可应用于园林美化。虽然早在2001年，我国广州和云南等地就开始进行帝萝花等山龙眼科木本花卉的资源引进和适应性研究（蹇洪英等，2006;苏开君等，2008），但由于繁殖效率低，因此导致其产品主要依靠进口，在市场上仍然还是“一枝独秀”。……