钟静 赵丽玲 李婷婷 丁铭

摘要 菜豆金色花叶病毒属病毒是一类在全球热带及亚热带地区造成严重经济损失的植物病毒，田间杂草是这类病毒重要的中间寄主。本研究从云南省玉溪市采集了表现黄脉的青蒿植株，通过PCR扩增、克隆及测序从样品中获得两条菜豆金色花叶病毒属病毒DNA-A全基因组序列，分别为YN6393-23和YN6393-27，其全長均为2 739 bp，相似性为100%。序列分析发现，YN6393-23和YN6393-27的核苷酸序列与烟草曲茎病毒Tobacco curly shoot virus（TbCSV）的分离物YN4584的核苷酸序列相似性最高，为99.45%。根据国际病毒分类委员会对菜豆金色花叶病毒属病毒种的分类标准，全基因组序列相似性大于91%则为同种病毒，表明此病毒分离物为烟草曲茎病毒的一个分离物。这是菜豆金色花叶病毒属病毒侵染青蒿植株的首次报道。

关键词 青蒿; 烟草曲茎病毒; 杂草

中图分类号： S 432.1

文献标识码： A

DOI： 10.16688/j.zwbh.2020297

Molecular identification and genomic structural characteristics analysis of Tobacco curly shoot virus infecting Artemisia caruifolia

ZHONG Jing#， ZHAO Liling#， LI Tingting， DING Ming*

（Institute of Biotechnology and Germplasm Resources， Yunnan Academy of Agricultural Sciences， Key Laboratory

of Agricultural Biotechnology of Yunnan Province， Key Laboratory of the Southwestern Crop Gene Resources and

Germplasm Innovation， Ministry of Agriculture and Rural Affairs， Kunming 650223， China）

Abstract

Begomoviruses in the family of Geminiviridae have emerged as a major constraint on crop production in tropical and subtropical regions， and weeds can be the intermediate hosts of begomoviruses. Artemisia caruifolia samples with yellow vein symptom were collected in Yuxi， Yunan province. Among which， the DNA-A complete genome sequence of two isolates （YN6393-23 and YN6393-27） belong to Begomovirus  were obtained  by PCR， cloning and sequencing. The full length of two sequences was 2 739 bp， with the sequence identity of 100%. Sequence analysis showed that these begomoviruses sequences shared the highest identity （99.45%） with Tobacco curly shoot virus YN4584. Based on the ICTV criteria for Begomovirus species demarcation （≥91% sequence identity for the complete genome）， the virus was identified as an isolate of TbCSV. This is first report that Artemisia caruifolia plant can be infected by begemovirus.

Key words

Artemisia caruifolia; Tobacco curly shoot virus; weed

双生病毒的病毒粒体为双联体结构，包裹着单分子或双分子环状单链DNA，DNA分子的长度在2.5～3.0 kb之间，双生病毒主要侵染双子叶植物，可危害棉花、木薯、番茄、烟草等多种经济作物[1-4]。近年来，我国广西、云南和海南等地陆续报道了多起因双生病毒危害而造成的经济损失事件，且双生病毒的危害范围及程度呈逐年上升的趋势[5-6]。在双生病毒科Geminiviridae的9个属中，种类最多（包含320多个种）、分布最广（亚洲、非洲及拉丁美洲等地均有分布）、危害最大的属是菜豆金色花叶病毒属Begomovirus。从基因组结构上看，既有双组分病毒又有单组分病毒是该属病毒的一个典型特征……