孙晶　郭阁　于堂　张璇

[关键词] 大鼠;β-防御素-2;牙龈上皮;中药

[中图分类号] R781.4          [文献标识码] A          [文章編号] 1673-9701（2021）22-0032-03

Screening of Chinese herbal medicine for inducing expression of β-defensin-2 in gingival epithelium

SUN Jing1   GUO Ge1   YU Tang2   ZHANG Xuan3

1.Department of Children′s Stomatology， Changsha Stomatological Hospital， Changsha   410000， China; 2.Department of Stomatology， Ji′ning First People′s Hospital in Shandong Province， Jining   272000， China; 3.Department of Children′s Stomatology， the Second Affiliated Hospital of Jinzhou Medical University， Jinzhou   121000， China

[Abstract] Objective To screen out the Chinese herbal medicine which can induce the expression of β-defensin-2 in rat gingival epithelium. Methods 90 rats in our hospital from February 2019 to April 2020 were selected as the research objects. Ninety healthy adult Wistar rats were randomly divided into three groups： the inherent expression group （10 rats）， the negative control group （10 rats） and the positive control group （10 rats）. The experimental group were given drugs respectively （the astragalus membranaceus group， the Radix Isatidis group， the salvia miltiorrhiza group， the radix bupleuri group， the herba houttuyniae group， and the andrographolide lactone group， with 10 rats in each group， a total of 60 rats）. After 7 days of continuous administration， the rats were sacrificed， and the gingival epithelium of the rats was cut. The expression of β-defensin-2 protein in the gingival epithelium of the rats was detected by the immunohistochemical method. Results The expressions of β-defensin-2 protein in the gingival epithelium of rats in the astragalus membranaceus group and the radix isatidis group were significantly higher than that in the inherent expression group， and the differences were statistically significant（P<0.05）， but no statistically significant differences were observed compared with the positive control group（P>0.05）. Conclusion Astragalus membranaceus and radix isatidis can induce the increase of β-defensin-2 expression in the gingival epithelium of rats， suggesting that astragalus membranaceus and radix isatidis can regulate the innate immunity of oral mucosa of rats.

[Key words] Rat; β-defensin-2; Gingival epithelium; Chinese herbal medicine

现今因抗生素滥用而引起的病原微生物耐药、过敏反应、正常菌群失调等问题日趋严重，研究和开发内源性肽类抗生素已备受医学界关注。防御素是现今研究中最受关注的内源性肽类抗生素分子，具有抗细菌、抗病毒、抗真菌、抗肿瘤等多种生物学活性，并且尚未发现有耐药菌的产生[1-2]。哺乳动物类防御素按其结构不同可分为α-防御素、β-防御素和θ-防御素三种。在哺乳动物口腔中，β-防御素主要分布于上皮细胞内，是宿主口腔天然免疫系统的重要组成部分，与多种口腔感染性疾病密切相关。研究表明，β-防御素受LPS、TNF-α、IL-1α、IL-1β等炎症因子的诱导表达[3-5]，而用于治疗感染性疾病的中药能够增强机体免疫能力，但其作用机制尚不明确，把β-防御素作为筛选抗菌中药或其有效成分作为新的研究方向，可以增加中药药效的物质基础，甚至还可能为解释中药抗感染机制提供新的依据[6]。本实验期望筛选出相关中药，可以为β-防御素-2基因表达的研究提供实验依据，现报道如下。