左玲 刘宏伟 朱宇珍

摘要： 目的 觀察黄芩汤对湿热型溃疡性结肠炎（UC）模型大鼠结肠组织中NF-κB p65和ICAM-1表达的影响，探讨黄芩汤治疗UC的作用机理。方法 将80只SD大鼠随机分为正常对照组、模型组、黄芩汤组、柳氮磺吡啶组，除对照组外，采用高脂高糖辛辣饮食加2，4，6三硝基苯磺酸（TNBS）诱导法构建湿热型UC大鼠模型，造模成功后，分别给予20 mL·kg-1体质量的黄芩汤和8%浓度的柳氮磺砒啶混悬液灌胃治疗，正常对照组和模型组给予等量的生理盐水，连续治疗2周，实时荧光定量聚合酶链式反应检测结肠组织中NF-κB p65和ICAM-1 mRNA表达。免疫组化检测结肠组织中NF-κB p65和ICAM-1蛋白表达。结果 与正常对照组比较，模型组NF-κB p65和ICAM-1 mRNA 及蛋白表达量明显升高（P<0.05）;与模型组比较，黄芩汤组和柳氮磺砒啶组NF-κB p65和ICAM-1 mRNA 及蛋白表达量显著下降（P<0.05）;黄芩汤组NF-κB p65和ICAM-1 mRNA表达量及蛋白阳性表达率显著低于柳氮磺砒啶组，2组比较差异具有统计学意义（P<0.05）。结论 黄芩汤对湿热型溃疡性结肠炎的治疗作用可能是通过下调NF-κB p65、ICAM-1的表达来实现。

关键词： 黄芩汤;溃疡性结肠炎;核因子κB;细胞间粘附分子-1

中图分类号：R516.1   文献标志码：A   文章编号：1007-2349（2021）08-0079-05

Effect of Scutellaria Baicalansis Decoction on the Expression of NF-κB p65 and ICAM-1in the Colon Tissue of Rats with Damp-Heat Ulcerative Colitis

ZUO Ling1， LIU Hong-wei2， ZHU Yu-zhen3

（1. The Second Affiliated Hospital of Guangdong Medical University， Zhanjiang 524003， China;  2. The First Clinical School of Guangdong Medical University， Zhanjiang 524001， China;  3. Key Laboratory of Guangdong Natural Medicine Research and Development， Zhanjiang 524023， China）

【Abstract】Objective： To observe the effect of Scutellaria baicalansis Decoction on the expression of NF-κB p65 and ICAM-1 in the colon tissue of a rat model of damp-heat ulcerative colitis （UC）， and to explore the mechanism of Scutellaria Decoction in treating UC. Methods： 80 SD rats were randomly divided into normal control group， model group， Scutellaria Decoction group and sulfasalazine group. Except for the control group， a high-fat， high-sugar spicy diet plus 2， 4， 6 trinitro-benzene sulfonic acid （TNBS） were used to construct a damp-heat UC rat model. After the model was successfully established， the rats were given by intragastric administration of 20 mLokg-1 body weight Scutellaria Decoction and 8% concentration of sulfasalazine suspension. The normal control group and the model group were given the same amount of normal saline for 2 successive weeks and the real-time fluorescence quantitative polymerase chain reaction was used to detect the expression of NF-κB p65 and ICAM-1 mRNA in the colon tissue and the immunity class chemically detected the NF-κB p65 and ICAM-1 protein expression in the colon tissue. Results： Compared with that of the normal control group， the NF-κB p65 and ICAM-1 mRNA and protein expression quantity of the model group was significantly increased （P<0.05）; compared with that of the model group， the NF-κB p65and ICAM-1 mRNA and protein quantity expression of the Scutellaria Decoction group and the sulfasalazine group decreased significantly （P<0.05）， The NF-κB p65 and ICAM-1 mRNA expression quantity and protein positive expression rate of the Scutellaria Decoction group was significantly lower than that of the sulfasalazine group. The difference was statistically significant between the two groups （P<0.05）. Conclusion： The curative effect of Scutellaria Decoction on damp-heat ulcerative colitis may be achieved by down-regulating the expression of NF-κB p65 and ICAM-1.

【Key words】Scutellaria Decoction; Ulcerative Colitis; Nuclear Factor κ B; Intercellular Adhesion Molecule-1

溃疡性结肠炎（Ulcerative colitis，UC）作为炎症性肠病（Inflammatory bowel disease，IBD）的一个亚型，主要特征是直肠、结肠的特发性慢性炎症[1]，与结肠癌的发生有一定的关系，容易反复发作，临床上难以根治[2]。笔者前期通过高脂高糖辛辣饮食加免疫复合法成功构建出湿热型UC模型，证实了黄芩汤能够显著改善湿热型UC大鼠的症候学、促进结肠病变黏膜修复[3]。UC的病因复杂，发病机制尚未完全阐明，研究表明NF-κB广泛参与炎症相关因子的转录调控[4]，直接调控细胞间粘附分子-1（ICAM-1）的转录，与UC的发生、发展密切相关[5]，但是黄芩汤是否对NF-κB和ICAM-1发挥调控作用尚未完全阐明。本研究旨在探讨黄芩汤对湿热型UC大鼠NF-κB和ICAM-1表达的影响，阐明其治疗湿热型UC的作用机制，现报道如下。……