黄新宇 陈丽 周忠志 胡晋婷 王霄霜 杨丁丁 王巍 叶永鑫 袁忠行 丁雅容 禚昭钦 刘平安

〔摘要〕 目的 從调节整合素β1、α-SMA探究仙藕乳蒲方促小鼠皮肤移植创面愈合的机制。方法 选用40只雌性C57BL/6小鼠，制作背部直径为1.2 cm圆形全层皮肤缺损模型，后进行自体原位皮肤移植，随机分为对照组和实验组，实验组给予仙藕乳蒲方粉末敷于皮肤移植区，对照组不予处理。免疫组织化学染色法比较组织整合素β1、α-SMA的含量;Masson染色比较两组皮肤移植区胶原纤维分布。结果 （1）免疫组化统计结果示：皮肤移植14 d，实验组整合素β1、α-SMA表达明显高于对照组（P<0.05）;皮肤移植21 d，实验组整合素β1、α-SMA表达则低于对照组（P<0.05）。（2）Masson染色显示：与对照组相比，皮肤移植14 d，实验组真皮层胶原纤维丰富，排列致密有序;皮肤移植21 d，实验组真皮层胶原纤维更少沉积，且排列疏松规则。结论 仙藕乳蒲方在皮肤移植创面愈合早期，可提高整合素β1、α-SMA的表达，加强胶原纤维的合成分泌并加快创面收缩;在创面愈合晚期，降低整合素β1、α-SMA的表达，减少胶原纤维的合成分泌并防止创面过度收缩。仙藕乳蒲方可能通过双向调节整合素β1、α-SMA的表达，发挥促进皮肤移植创面愈合及降低纤维化的作用，为仙藕乳蒲方运用于临床皮肤移植创面愈合提供依据。

〔关键词〕 仙藕乳蒲方;皮肤移植;创面愈合;整合素β1;α-SMA

〔中图分类号〕R285.5        〔文献标志码〕A       〔文章编号〕doi：10.3969/j.issn.1674-070X.2021.07.008

〔Abstract〕 Objective To investigate the mechanism of the Xian'ou Rupu Recipe promoting the healing of mouse skin graft wounds by regulating integrin β1 and α-SMA. Methods 40 female C57BL/6 mice were used to make a circular full-thickness skin defect model with a diameter of 1.2 cm on the back， autologous orthotopic skin graft were performed and divided into control group and experimental group. In the experimental group， the Xian'ou Rupu Recipe powder was applied to the skin grafting area， and the control group was not treated. Immunohistochemical staining was used to compare the content of integrin β1 and α-SMA; Masson staining was used to compare the distribution of collagen fibers in the skin graft area between the two groups. Results （1） The statistical results of immunohistochemistry showed that the expression levels of integrin β1 and α-SMA in the experimental group were significantly higher than that in the control group at 14 days after skin transplantation （P<0.05）; at 21 days after the skin transplantation， the expression levels of integrin β1 and α-SMA in the experimental group were lower than that in the control group （P<0.05）. （2） Masson staining showed that compared with the control group， 14 days after skin transplantation， the experimental group had rich collagen fibers in the dermis layer， and the arrangement was dense and orderly; 21 days after skin transplantation， the experimental group had less deposition of collagen fibers in the dermis layer， and the arrangement was loose and regular. Conclusion The Xian'ou Rupu Recipe can increase the expression of integrin β1， α-SMA， strengthen the synthesis and secretion of collagen fibers and accelerate the contraction of wounds in the early stage of skin graft wound healing; in the late stage of wound healing， reduce the expression of integrin β1， α-SMA， reduce synthesis and secretion of collagen fibers and prevent excessive contraction of the wound surface. It was confirmed that the Xian'ou Rupu Recipe may play a role in promoting skin graft wound healing and reducing fibrosis by bidirectionally regulating the expression of integrin β1 and α-SMA， providing a basis for the application of the Xian'ou Rupu Recipe in clinical skin graft wound healing.

〔Keywords〕 Xian'ou Rupu Recipe; skin transplantation; wound healing; integrin β1; α-SMA

皮肤移植是一种广泛应用于临床的有效治疗手段，但如何提高植皮后创面愈合率仍需进一步探索[1-2]。移植创面愈合是涉及细胞与细胞外基质（extracellular matrix， ECM）相互作用的动态的复杂过程，整合素β1是整合素家族的重要成员，通过“ECM-整合素-肌动蛋白骨架”系统参与多种细胞生物学进程，如细胞信号传导、细胞黏附与迁移等[3-4]。α-平滑肌肌动蛋白（α-smooth muscle actin， α-SMA）是细胞骨架的重要组分之一，在整合素胞内端传递的信号作用下，发生聚合或解聚引起细胞骨架构象的改变，影响细胞迁移运动、胶原分泌及组织收缩[5-6]。

仙藕乳蒲方基于益气活血、祛腐生新而立方，由仙鹤草、藕节、乳香和蒲黄按君臣佐使原则精密配伍而成，中医学认为其具有收敛止血、活血祛瘀、消肿生肌的功效[7]，是临床治疗创伤疗效显著的经验方。……