苦瓜控制第1雄花节位的QTL定位
2021-08-23崔竣杰彭家柱程蛟文胡开林
崔竣杰 彭家柱 程蛟文 胡开林



摘 要:为了对控制苦瓜第1雄花节位的遗传位点进行定位,基于苦瓜K44×Dali-11的F2群体和04-17×47-2-1-1-3的F2群体构建遗传图谱,结合第1雄花节位的表型数据,对苦瓜第1雄花节位进行了QTL检测。结果表明,对于2个F2群体,都在相近的遗传位置区域检测到控制苦瓜第1雄花节位的QTL位点fmfn,该位点位于苦瓜的MC06连锁群或拟染色体上10.66~14.32 Mb区间,候选区间的遗传距离和物理距离分别为11.65 cM和3.66 Mb,遗传贡献率为34.80%,其中包含195个注释基因。研究结果为进一步精细定位及克隆控制苦瓜第1雄花节位的基因提供了重要参考依据。
关键词:苦瓜;第1雄花节位;QTL;候选区间
中图分类号:S642.5 文献标志码:A 文章编号:1673-2871(2021)07-025-04
QTL mapping of the first male flower node in bitter gourd
CUI Junjie1, PENG Jiazhu2, CHENG Jiaowen3, HU Kailin3
(1. Department of Horticulture, Foshan University, Foshan 528000, Guangdong, China; 2. Guangzhou Academy of Agricultural Sciences, Guangzhou 510335, Guangdong, China; 3. College of Horticulture, South China Agricultural University, Guangzhou 510642, Guangdong, China)
Abstract: In order to map the genetic locus controlling the first male flower node of bitter gourd, based on the genetic maps constructed by the F2 populations of K44×Dali-11 and 04-17×47-2-1-1-3, combined with the phenotypic data of the first male flower node, we detected QTLs for the first male flower node of bitter gourd. The results showed that for the two F2 populations, the QTL locus, fmfn, controlling the first male flower node of bitter gourd was detected in the similar genetic location, which was located in the 10.66-14.32 Mb interval on the linkage group or pseudo chromosome MC06. The genetic and physical distance of the candidate interval are 11.65 cM and 3.66 Mb, respectively, and the genetic contribution rate is 34.80%, including 195 annotated genes. The results of this study provide an important reference for further fine mapping and cloning of genes controlling the first male flower node of bitter gourd.
Key words: Bitter gourd; First male flower node; QTL; Candidate interval
苦瓜(Momordica charantia L.)属于葫芦科作物,主要分布在亚洲和非洲[1],通常为单性花,雌雄同株,雌花接受雄花花粉授粉后果实才迅速发育和膨大,因此苦瓜开花节位的高低会显著影响苦瓜的熟性和早期产量。
葫芦科作物的性别分化既受环境的影响,又受遗传的调控。在环境因素方面,光照和温度对苦瓜植株性别分化的影响与黄瓜类似,即短日照和低温条件下有利于雌花形成[2]。在遗传因素方面,国内外学者对葫芦科作物的性别决定、始花节位和早开花等开花相关性状进行了一系列的研究。例如明确了甜瓜植株性别形态主要取决于3个基因位点(M位点、G位点和A位点)的显隐性组合[3];利用SRAP(sequence-related amplifiedpolymorphism)标记将黄瓜的始花节位基因ffn定位于Ⅸ连锁群上标记DC1EM5-ME7EM2A的1.80 cM区间[4];利用SSR(simple sequence repeats)标记将黄瓜第1雌花节位2个QTL(Fpfn 3.1和Fpfn 6.1)分别定……
