王广宇　　陈潇潇　　侯显良

[關键词] 原发性胆汁性胆管炎;16S rDNA;肠道菌群;高通量测序

[中图分类号] R575.2          [文献标识码] B          [文章编号] 1673-9701（2021）19-0139-05

Detecting changes of intestinal microflora in patients with primary biliary cholangitis based on 16S rDNA sequencing

WANG Guangyu1   CHEN Xiaoxiao2   HOU Xianliang1

1.Central Laboratory， Guangxi Health Commission Key Laboratory of Glucose and lipid Metabolism Disorders， the Second Affiliated Hospital of Guilin Medical University， Guilin   541199， China; 2.State Key Laboratory for Diagnosis and Treatment of Infectious Diseases， the First Affiliated Hospital， College of Medicine，Zhejiang University， Hangzou   310006， China

[Abstract] Objective To study the abundance and diversity of intestinal flora in Primary biliary cholangitis （PBC） patients. Methods Nine patients with primary biliary cholangitis addmitted in the First Affiliated Hospital of Zhejiang University Medical College from January 2018 to January 2019 were selected as research subjects. Another 8 healthy subjects were selected as healthy controls. The intestinal flora DNA was extracted， and the corresponding gene fragments of V3-V4 region were amplified by high-throughput 16S rDNA sequencing， and the database was constructed for sequencing and bioinformatics analysis. Results In Alpha diversity analysis， there were no significant differences in ACE index， Chao index， Simpson index and Shannon index between the two groups（P>0.05）. Through OTU clustering and species annotation， we found that there were statistical differences in the abundance of some flora between the two groups （P<0.05）. Compared with healthy control group（NC）， Cellulosilyticum， Holdemanella， Lachnospiraceae， Lactococcus and Morganella were decreased and Hungatella was significantly increased in PBC group. In addition， real-time quantitative PCR was used to detect the content of intestinal Escherichia coli in the PBC group and the NC group， and it was found that the content in the PBC group was significantly higher than that in the NC group （P<0.05）. Conclusion Compared with healthy control group（NC）， the primary biliary cholangitis（PBC）group shows changes in intestinal flora to a certain extent. Further analysis of changes in intestinal flora is expected to provide new ideas for the study of the occurrence and development of primary biliary cholangitis and the search for new sensitive microbial markers.