牛延菲 王鹏 张晓南 徐怡 曹红云 苏钛

摘要：目的 对雪上一枝蒿中多糖的含量测定方法进行研究，建立紫外分光光度法测定雪上一枝蒿多糖的含量测定方法，并考察不同产地、不同炮制方式对多糖含量的影响。方法 用80%乙醇回流去除还原性糖等杂质，后热水提取多糖，采用苯酚-硫酸比色法于488 nm波长处用紫外-分光光度法测得吸光度值，从而得出其多糖的含量。结果 雪上一枝蒿多糖以无水葡萄糖计，在0.003～0.015 mg/mL范围内呈良好的线性关系（R=0.9999），回收率为99.35%～99.85%（n=6），RSD值为0.18%。结论 本法准确、可靠，重现性好，适用于测定雪上一枝蒿中多糖含量;不同产地不同批次药材中的多糖含量存在一定差异;炮制方式对雪上一枝蒿多糖含量无显著影响。

关键词：雪上一枝蒿;多糖;紫外分光光度法;产地;炮制

中图分类号：R285.5 文献标志码：A 文章编号：1007-2349（2021）05-0064-06

【Abstract】Objective： To observe the expression of HSP60 and Caspase-3 of acupuncture in rats with cerebral infarction and to preliminarily explore the protective mechanism of acupuncture on neuronal apoptosis in rats with ischemic stroke. Methods： The cerebral ischemia rats were induced by middle cerebral artery suture to make reperfusion injury models and randomly divided into sham operation group， model group， electroacupuncture point group， electroacupuncture non-meridian non-acupoint group and moxibustion acupoint group. After the intervention of electroacupuncture or moxibustion， the neurological deficit score and postural reflex score of the rats were evaluated at 0 and 12 hours after the operation. The expression levels of HSP60 and Caspase-3 mRNA were detected by fluorescence quantitative PCR. Results： The nerve function of the rats in the electro-acupuncture acupoint group and moxibustion acupoint group was better than that of the model group， and the difference was statistically significant（P<0.05）. Compared with that of the model group， the HSP60 mRNA of the electro-acupuncture acupoint group increased significantly， and the difference was significant（P<0.05） while the expression of Caspase-3 mRNA did not change significantly. Conclusion： Electroacupuncture can improve the neurological function of rats with cerebral ischemia-reperfusion injury. The mechanism may be related to the up-regulation of the expression of HSP60 in brain tissue and mediating anti-neuronal cell apoptosis.

【Key words】Ischemic Stroke; HSP60; Caspase-3; Nerve Cell Apoptosis

雪上一枝蒿為毛茛科植物短柄乌头Aconitum brachypodum Diels.的干燥块根[1]。其味苦、辛，性温，有大毒，主产于云南北部的东川、会泽等地，具有祛风镇痛的功效，用于治疗风湿疼痛、跌打损伤等症。雪上一枝蒿含有乌头碱、次乌头碱、雪上一枝蒿甲素等多种生物碱和β-谷甾醇、胡萝卜苷等非生物碱成分，其中生物碱主要是二萜生物碱，目前认为是其主要成分，雪上一枝蒿具有镇痛、局部麻醉、抗炎和抗肿瘤等药理作用[2]。以雪上一枝蒿为原料，开发了包括雪上一枝蒿注射剂、骨痛灵酊、云南白药气雾剂、云南白药膏、肿痛气雾剂等多种制剂，是云药产业发展中一个很具特色的品种。……