一株降解苯酚的翅碱蓬内生菌降解条件优化
2021-07-25刘慧敏韦琳娜张子康张孝霖李玉玺
刘慧敏 韦琳娜 张子康 张孝霖 李玉玺



摘 要:苯酚是重要的有机合成原料,但具有毒性,对环境和生物体具有较强的毒害作用。用生物法降解苯酚具有成本低、效率高、二次污染少等优点。为提高微生物降解苯酚的效率,对从黄河三角洲盐碱地生翅碱蓬中分离出的一株能够有效降解苯酚的菌株进行降解条件优化试验,得到该菌株的最佳培养时间为36h、最适pH为7、苯酚降解最适初始浓度为500mg/L。
关键词:苯酚降解菌;翅碱蓬;降解条件;优化
中图分类号 X172文献标识码 A文章编号 1007-7731(2021)12-0019-02
Optimization of Degradation Conditions of a Phenol Degrading Endophytic Bacterium from Phragmites Australis
LIU Huimin et al.
(School of Biology and Environmental Engineering, Binzhou University, Binzhou 256603, China)
Abstract: Phenol is an important raw material for organic synthesis, but because of its toxicity, it has a strong toxic effect on the environment and organisms. Biodegradation of phenol by biological method has the advantages of low cost, high efficiency and less secondary pollution . In order to improve the efficiency of phenol degradation by microorganism, this paper studied and optimized the degradation conditions of a strain which could effectively degrade phenol from Suaeda salsa in saline alkali soil of Yellow River Delta. The optimal culture time of the strain was 36h, the optimum pH value was 7, and the optimal initial phenol degradation was obtained The concentration was 500mg/L.
Key words: Phenol degrading bacteria; Suaeda salsa; Degradation conditions; Optimization
苯酚是有机合成中的基础原料,被广泛应用于各行各业中[1,2]。但苯酚的毒性很大,仅通过皮肤接触就可能对人体产生致命性的伤害,2017年苯酚还被世界卫生组织列入第三类致癌物清单[3-6]。但由于苯酚属于基础性原料,应用范围广,可替代性低,在某些工业生产中必不可少,这就导致含有大量苯酚的工业污水、废气排放到环境中,对环境和人体健康都造成了严重危害。目前,清除工业废水中苯酚的方法主要有物理法、化学法、生物法三大类。生物法降解苯酚是指某些生物体内含有特殊的酶系,能以苯酚作为生长的唯一碳源与能源,进而在生长代谢过程中降解苯酚。与物理、化学方法相比,生物法成本低、效率高,且不会造成二次污染,更适合大规模应用于环境治理[7,8]。为此,笔者对从黄河三角洲盐碱地生翅碱蓬中分离出的能够有效降解苯酚的菌株进行试验,确定降解苯酚类污染物的菌株的最适降解条件,为今后更好地应用生物法降解苯酚提供参考。……
