刘晴 徐阳 张妍 丁琳琳 潘丕克

摘 要：土壤酶活性是土壤肥力的重要指标之一，为探究抚育间伐对辽东山区红松人工林林下不同土层酶活性的影响，采用紫外分光光度法、靛酚蓝比色法、3，5- 二硝基水杨酸比色法和磷酸苯二钠比色法分别测定不同间伐程度林地土壤表层（0～10 cm）和亚表层（≥10～20 cm）不同酶的活性。结果表明：林下土壤酶活性表现为表层大于亚表层;过氧化氢酶活性在弱度区和中度区的表层差异显著，中度间伐酶活性较高，对酶活性影响最大;脲酶在弱度区差异显著，弱度间伐对酶活性影响较大;酸性磷酸酶的活性在中度区变化较大且差异显著;蔗糖酶在表层弱度和中度间伐差异显著，且中度区相对其他间伐处理酶的活性较高。在辽东山区红松人工林中，弱度间伐及中度间伐对林下土壤酶活性影響较大。

关键词：红松人工林;抚育间伐;脲酶;蔗糖酶;酸性磷酸酶;过氧化氢酶

中图分类号：S718.5    文献标识码：A   文章编号：1006-8023（2021）03-0067-05

Abstract：Soil enzyme activity is one of the important indicators of soil fertility. In order to explore the effects of thinning on soil enzyme activities of Pinus koraiensis plantation in Liaodong mountain area in different soil layers under the forest， in this paper， the surface （0～10 cm） and subsurface （≥10～20 cm） soil enzyme activities of different thinning degrees were determined by UV spectrophotometry， indophenol blue colorimetry， 3，5dinitrosalicylic acid colorimetry and disodium phenylphosphate colorimetry. The results showed that： the soil enzyme activities in the surface layer were higher than that in the subsurface layer. The activity of catalase was significantly different in the surface layer between the weak area and the moderate area. The activity of catalase was higher in the moderate area， which had the greatest impact on the activity of catalase. Urease showed significant difference in weak degree area， and weak thinning had a greater impact on urease activity. Acid phosphatase showed a large and significant difference in enzyme activity in the moderate zone. Sucrase showed significant difference in surface weak degree and moderate thinning， and the enzyme activity in moderate area was higher than that in other thinning treatments. In Pinus koraiensis plantation in Liaodong mountain area， weak thinning and moderate thinning had a greater impact on soil enzyme activities.

Keywords：Pinus koraiensis plantation; thinning; urease; sucrase; acid phosphatase; catalase

0 引言

红松作为我国东北东部山地地带性植被的优势树种，用途广泛，具有较高的经济价值和生态价值。辽宁省森林经营研究所实验林场拥有自1949年营造的新中国第一处红松人工林，到2020年林龄达71 a，是具有代表性的红松人工林，对其研究具有重要意义。

土壤中的酶是林下土壤中最活跃的有机成分之一，参与土壤能量流动和物质循环，土壤中进行的化学和生物反应也是在酶的催化下进行的，因此酶是生态系统代谢中重要驱动力[1-2]。……