赛乌其日 新吉乐 特格喜白音 敖登其木格 乌力吉特古斯

摘要 目的：利用制备型高效液相色谱（PHPLC）研究草乌叶生物碱的分离纯化工艺为目的。方法：利用苯提取法得到草乌叶苯提取物。利用PHPLC将草乌叶苯提取物进行初步分离，分离产物用薄层色谱和分析型液相色谱分析，以确定目标峰。再次用PHPLC对目标峰进行分离和纯化，对所得各峰进行收集，薄层色谱和分析型液相色谱分析分离产物，验证分离效果。结果：草乌叶苯提取物经PHPLC初步分离后得到峰1～峰12。经薄层色谱和分析型液相色谱分析得知峰5～峰10含有大量生物碱。选择峰5作为研究对象，进一步使用PHPLC进行分离纯化，得到峰5-1～峰5-12等12个峰。薄层色谱和分析型液相色谱分析结果证明，峰5-4基本包含了峰5内的生物碱，为目标分离产物。结论：PHPLC初步分离和二次分离建立的色谱条件，可为草乌叶生物碱的大量精制提供依据。

关键词 草乌叶;生物碱;制备型高效液相色谱

A study of Alkaloids Separation and Purification Method from Leaves of Kusnezoff Monkshood Root

Saiwuqiri1，Xinjile2，Tegexibaiyin2，Aodengqimuge2，Wulijitegusi2

（1 Hang Jin banner Mongolian Medicine General Hospital，Inner Mongolia Autonomous Region，Ordos 017400，China;

2 Inner Mongolia International Mongolian Medicine Hospital，Hohhot 010010，China）

Abstract Objective：To study the alkaloids separation and purification method from leaves of kusnezoff monkshood root using Preparative High Performance Liquid Chromatography（PHPLC）.Methods：benzene extraction method was used to obtain benzene extract from leaves of kusnezoff monkshood root.PHPLC was used to separate ingredients of benzene extract from leaves of kusnezoff monkshood root.Thin layer chromatography（TLC）and High Performance Liquid Chromatography（HPLC）were used to analyze the separated ingredients and determine the target peak.PHPLC was used to purify the target peak，the peaks were collected，the separated products were analyzed by TCL and HPLC to verify the separation effect.Results：A total of 12 Peaks were collected using PHPLC separation，designated as Peak 1～Peak 12.TLC and HPLC analysis showed that Peak 5～Peak 10 were enriched in alkaloids.Peak 5 was selected as an example to receive further PHPLC separation.A total of 12 peaks were seen，defined as Peaks 5-1～Peak 5-12.TLC and HPLC illustrated that Peak 5-4 contained most of alkaloids in Peak 5，which was the target separation product.Conclusion：Chromatographic conditions established by PHPLC primary separation and secondary separation can provide basis for the large-scale extraction of alkaloids from leaves of kusnezoff monkshood root.

Keywords Leaves of kusnezoff monkshood root; Alkaloids; Preparative high performance liquid chromatography

中圖分类号：R284.1文献标识码：Adoi：10.3969/j.issn.1673-7202.2021.02.006

草乌叶为毛莨科植物北乌头Aconitum kusnezoffii Reichb.的干燥叶。其主要成分为生物碱，草乌中起治疗作用和毒性作用的化合物均为乌头型生物碱。为常用蒙医药，《中华人民共和国药典》和《内蒙古蒙药材标准》均收载，有清热、解毒和止痛等功效，用于热病发烧、泄泻腹痛、头痛牙痛等。本研究利用制备型高效液相色谱仪分离纯化草乌叶生物碱，为了得到纯度较高的草乌叶生物提取方法碱奠定基础。

1 仪器与试药

1.1 仪器 隔膜真空泵（天津津腾……