毕建钢 李琪 郭煜升 唐鸿贵 鲍世韵

【摘要】 目的：探究與分析长链非编码RNA SNHG20（lncRNA SNHG20）在肝癌组织中的表达和临床意义。方法：选取2019年10月-2021年1月本院收治的90例肝癌患者，检测肝癌组织和癌旁组织lncRNA SNHG20的表达，检测肝癌组织和癌旁组织β-Catenin、Cyclin D1及C-myc基因的表达，采用Pearson相关性分析lncRNA SNHG20与β-Catenin、Cyclin D1及C-myc表达的相关性。分析肝癌患者不同微血管侵犯、分化程度及TNM分期情况下lncRNA SNHG20的表达差异。结果：肝癌组织lncRNA SNHG20、β-Catenin、Cyclin D1及C-myc的表达均显著高于癌旁组织（P<0.05）。Pearson相关性分析显示，lncRNA SNHG20与β-Catenin、CyclinD1及C-myc的表达均呈正相关（P<0.05）。不同微血管侵犯、分化程度及肿瘤分期患者病灶组织lncRNA SNHG20、β-Catenin、Cyclin D1及C-myc表达显著不同，差异均有统计学意义（P<0.05）。结论：lncRNA SNHG20在肝癌组织中呈高表达状态，其在不同微血管侵犯情况、分化程度及肿瘤分期者中的差异较大。

【关键词】 长链非编码RNA SNHG20 肝癌组织 微血管侵犯 分化程度 肿瘤分期

Expression and Clinical Significance of Long Non Coding RNA SNHG20 in Tissues of Hepatocellular Carcinoma/BI Jiangang， LI Qi， GUO Yusheng， TANG Honggui， BAO Shiyun. //Medical Innovation of China， 2021， 18（24）： -161

[Abstract] Objective： To investigate and analyze the expression and clinical significance of long non coding RNA SNHG20 （lncRNA SNHG20） in tissues of hepatocellular carcinoma. Method： A total of 90 hepatocellular carcinoma patients treated in our hospital from October 2019 to January 2021 were collected， the expressions of lncRNA SNHG20 of tumor and adjacent normal tissue were detected， the expressions of β-Catenin， Cyclin D1 and C-myc protein gene of tumor and adjacent normal tissue were detected， and the differences of these expressions between tumor and adjacent normal tissue were compared. The correlation of the expressions of lncRNA SNHG20 with β-Catenin， Cyclin D1 and C-myc were analyzed with Pearson correlation analysis. The differences of expression of lncRNA SNHG20 with microvascular invasion，tumor differentiation degree and disease stages were analyzed with variance analysis. Result： The expressions of lncRNA SNHG20， β-Catenin， Cyclin D1 and C-myc in the tumor tissue were all significantly higher than those in the adjacent normal tissue （P<0.05）. Pearson correlation analysis showed the expression of lncRNA SNHG20 were significantly positive correlated with the expressions ofβ-Catenin， Cyclin D1 and C-myc protein （P<0.05）. The expressions of lncRNA SNHG20， β-Catenin， Cyclin D1 and C-myc of lesion tissue in patients with different microvascular invasion， differentiation degree and tumor stages were significantly different， the differences were statistically significant （P<0.05）. Conclusion： lncRNA SNHG20 in hepatocellular carcinoma tissues is significantly higher than that in adjacent normal tissue， the expression of lncRNA SNHG20 is significantly correlated with microvascular invasion situation， differentiation degree and tumor stages.

[Key words] Long non coding RNA SNHG20 Tissues of hepatocellular carcinoma Microvascular invasion Differentiation degree Tumor stages

First-author’s address： Shenzhen People’s Hospital （Second Clinical School of Ji’nan University， First Affiliated Hospital of Southern University of Science and Technology）， Shenzhen 518020， China

doi：10.3969/j.issn.1674-4985.2021.24.039

肝癌作为在临床中较为常见的恶性肿瘤，危害极大，是临床研究的重点之一。临床中与肝癌相关的研究，病灶组织相关指标的表达研究是重点与热点[1-2]。另外，临床中近年来关于长链非编码RNA在各类恶性肿瘤中的研究不断增多，其中长链非编码RNA SNHG20（lncRNA SNHG20）即是研究较多的一类指标[3-5]，但是其在肝癌中的表达研究相对较少，同时在疾病相关方面，包括微血管侵犯情况、分化程度及肿瘤分期中的检测意义研究不足。因此，本研究就lncRNA SNHG20在肝癌组织中的表达和临床意义进行细致探究，以为肝癌的临床诊治提供理论基础。现报道如下。

1 资料与方法

1.1 一般资料 收集2019年10月-2021年1月本院收治的90例肝癌患者的肝癌组织、癌旁组织和临床信息。……